CD3 zeta Antibody (4B10)

Images

 
Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of K562 cells using anti-CD247 mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of human Thymus tissues using anti-CD247 mouse mAb
Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of Jurkat cells using anti-CD247 mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
4B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD3 zeta Antibody (4B10) Summary

Immunogen
Purified recombinant fragment of human CD247 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD247
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CD3 zeta Antibody (4B10)

  • CD247 antigen
  • CD247 antigen, zeta subunit
  • CD247 molecule
  • CD247
  • CD3 zeta
  • CD3H
  • CD3Q
  • CD3z antigen, zeta polypeptide (TiT3 complex)
  • CD3Z
  • CD3ZT-cell receptor T3 zeta chain
  • IMD25
  • T3Z
  • T-cell antigen receptor complex, zeta subunit of CD3
  • T-cell surface glycoprotein CD3 zeta chain
  • TCR Zeta Chain
  • TCRZCD3-ZETA

Background

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD3 zeta Antibody (NBP1-42561). (Showing 1 - 2 of 2 FAQ).

  1. I have recently ordered CD3 zeta antibody (4B10) Catalog number: NBP1-42561 Lot: 4310. According to the datasheet the concentration should be labeled on the vial. Unfortunately, I missed to find any concentrational detail on the vial, neither the concentration nor the amount. It would be grateful if you let me informed about the concentration.
    • The formulation for this product is ascites fluid, therefore the concentration is unknown as it is not purified antibody. There would be many other protein factors contained in the ascitic fluid making determination of an exact antibody concentration impossible without additional purification. The reason an isotype is known and provided is due to the fact that this is a monoclonal antibody and not because additional purifications were performed for that isotype.
  2. I would like to stain for human CD3 and IL12Rb2 antigen in human skin sections. Skin is PFA fixed and paraffin embedded. Will CD3 Ab (NBP1-42561) work on the sections after antigen retrieval?
    • NBP1-42561 has been validated in IHC-Pariffin sections for human tissue. As long as the target is expressed in your tissue, this antibody will work for detection. We generally recommend the Citrate buffer pH 6.0 antigen retrieval.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD247
Entrez
Uniprot