CD3 epsilon Antibody

Western Blot: CD3 epsilon Antibody [NBP2-29472] - Western Blot of Jurkat cell lysate using CD3 epsilon Antibody

Western Blot: CD3 epsilon Antibody [NBP2-29472] - Analysis of CD3e in human Jurkat lysate in the 1) absence and 2) presence of immunizing peptide, 3) on mouse thymus tissue lysate probed with 2 ug/ml of CD3e antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD3 epsilon Antibody Summary

• Description: 200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33116)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3 epsilon (Uniprot: P07766)
• Localization: Cell surface and cytoplasmic
• Marker: T-Cell Marker
• Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD3E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 1-2 ug/ml
• Application Notes: Western Blot: 1-2ug/ml for 60 minutes at RT.
  Use in Immunohistochemistry reported in scientific literature (PMID: 29867591).
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Ape, Orangutan, Gibbon, Chimpanzee, Monkey, Elephant, Equine, Bovine, Sheep, Porcine, Canine, Feline, Rabbit, Rat, Mouse, Guinea Pig, Hamster, Opossum, Frog, Chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for CD3 epsilon Antibody

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD3 epsilon Antibody (NBP2-29472)(1)

Publication using NBP2-29472

• Moes Arthur D, Severs David, Verdonk Koen et al. Mycophenolate Mofetil Attenuates DOCA-Salt Hypertension: Effects on Vascular Tone. Frontiers in Physiology 2018-01-01 [PMID: 29867591] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: CD3E
• Uniprot:
  • P07766()
  • P07766()
  • P07766()