CD3 epsilon Antibody


Western Blot: CD3 epsilon Antibody [NBP2-29472] - Analysis of CD3e in human Jurkat lysate in the 1) absence and 2) presence of immunizing peptide, 3) on mouse thymus tissue lysate probed with 2 ug/ml of CD3e antibody. more

Product Details

Reactivity Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, Eq, Fe, GP, Ha, Other, Pm, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Please see the vial label for concentration. If unlisted please contact technical services.

CD3 epsilon Antibody Summary

A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e
Cell surface and cytoplasmic
T-Cell Marker
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa.
Protein A purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS with 0.05% BSA.
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein A purified


  • Western Blot 0.5-1.0ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
Application Notes
Human Chromosome Location: 11q23.3
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
23 kDa

Reactivity Notes

Elephant, Gibbon, Macaque, Mole Rat, New World Monkey, Orangutan, Rhesus Monkey, Chimpanzee, Frog, Opossum and Ape.

Alternate Names for CD3 epsilon Antibody

  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE


The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol CD3E

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