TCR alpha Antibody (3A8)

• Reactivity: Hu, Mu(-), Rt(-)
• Applications: IHC, IHC-Fr, IP
• Clone: 3A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

TCR alpha Antibody (3A8) Summary

• Immunogen: Human TCR ALPHA
• Specificity: Reacts with the alpha chain constant region found on all alpha beta TCR bearing T-lymphocytes. It reacts with 92-98% of all CD3 + peripheral blood lymphocytes.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: It has been reported that this antibody does not work in Flow Cytometry.

Reactivity Notes

Reacts with Human. Does not react with Mouse or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A purified

Alternate Names for TCR alpha Antibody (3A8)

• C)
• D
• FLJ22602
• J
• MGC117436
• MGC22624
• MGC23964
• MGC71411
• T cell receptor alpha locus
• T-cell antigen receptor, alpha polypeptide
• T-cell receptor, alpha (V
• TCRA
• TCRD
• TRA

Background

The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCR alpha Antibody (NB100-65265)(1)

Publication using NB100-65265

• Bein J, Thurner L, Hansmann ML, Hartmann S Lymphocyte predominant cells of nodular lymphocyte predominant Hodgkin lymphoma interact with rosetting T cells in an immunological synapse Am. J. Hematol. Aug 20 2020 [PMID: 32815561] (IHC, Human)

Bioinformatics

• Gene Symbol: TRA
• Entrez:
  • 28755(Human)
  • 21473(Mouse)
  • 290071(Rat)
• Uniprot:
  • P01848(Human)