CD27/TNFRSF7 Antibody [Unconjugated]

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CD27/TNFRSF7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF382) at 3 µg/mL overnight at ...read more
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and human tonsil tissue. PVDF membrane was probed with 1 ...read more
Simple Western lane view shows lysates of human tonsil tissue, loaded at 0.2 mg/mL. A specific band was detected for CD27/TNFRSF7 at approximately 49 kDa (as indicated) using 20 µg/mL of Goat Anti-Human ...read more
In the presence of sub-optimal amounts of Mouse CD3 epsilon Monoclonal Anti­body (Catalog # MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recom­binant Human CD27 Fc Chimera (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CD27/TNFRSF7 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27
Thr21-Ile192
Accession # P26842
Specificity
Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CD27 is observed and less than 1% cross-reactivity with recombinant human (rh) CD30, rh4-1BB, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhHVEM, rhRANK, rhNGF R, rhsTNF RI, and rhsTNF RII is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD27
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 20 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • CyTOF-ready
  • Neutralization 2.25-9.0 ug/mL
Publications
Read Publications using
AF382 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD27/TNFRSF7 Antibody [Unconjugated]

  • CD27 antigen
  • CD27 molecule
  • CD27
  • MGC20393
  • S152CD27L receptor
  • T cell activation antigen CD27
  • T cell activation antigen S152
  • T14
  • TNFRSF7
  • TNFRSF7T-cell activation antigen CD27
  • Tp55
  • Tumor necrosis factor receptor superfamily member 7
  • tumor necrosis factor receptor superfamily, member 7

Background

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4+ compartment, it is preferentially expressed on CD45RA+ cells. In contrast, it is preferentially expressed on CD45RO+ cells in the CD8+ compartment. CD27 also appeaars to be a potential marker for memory B cells. It exists as both a disulfide-linked dimer on the cell surface and as a soluble protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 20 aa signal, a 173 aa extracellular domain, a 20 aa transmembrane domain, and a 47 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and a small subset of activated T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses.

  1. Camerini, D. et al. (1991) J. Immunol. 147:3165.
  2. Loenen, W.A. et al. (1992) J. Immunol. 149:3937.
  3. Lens, S.M.A. et al. (1998) Sem. Immunol. 10:491.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD27/TNFRSF7 Antibody (AF382)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development, WB.


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(1)
WB
(1)
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Bioinformatics

Gene Symbol CD27
Uniprot