CD27/TNFRSF7 Antibodies

HAF017). A specific band was detected for CD27/ TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for CD27/ TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF382) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CD27/ TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recom-binant Human CD27 Fc Chimera (Catalog # 382-CD) inhibits proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, inhibition of proliferation elicited by Recombinant Human CD27 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF382). The ND₅₀ is typically 2.25-9.0 µg/mL." class="big_lightbox"> MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recom-binant Human CD27 Fc Chimera (Catalog # 382-CD) inhibits proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, inhibition of proliferation elicited by Recombinant Human CD27 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF382). The ND₅₀ is typically 2.25-9.0 µg/mL." class="big_thumb" />

MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recombinant Human CD27 Fc Chimera (Catalog # 382-CD) inhibits proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, inhibition of proliferation elicited by Recombinant Human CD27 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD27 Monoclonal Antibody (Catalog # MAB382). The ND₅₀ is typically 0.2-0.4 µg/mL." class="big_lightbox"> MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recombinant Human CD27 Fc Chimera (Catalog # 382-CD) inhibits proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, inhibition of proliferation elicited by Recombinant Human CD27 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD27 Monoclonal Antibody (Catalog # MAB382). The ND₅₀ is typically 0.2-0.4 µg/mL." alt="In the presence of sub-optimal amounts of Mouse CD3e Monoclonal Anti-body (Catalog # MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recombinant Human CD27 Fc Chimera (Catalog # 382-CD) inhibits proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, inhibition of proliferation elicited by Recombinant Human CD27 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD27 Monoclonal Antibody (Catalog # MAB382). The ND₅₀ is typically 0.2-0.4 µg/mL." class="big_thumb" />

HAF005). A specific band was detected for CD27/TNFRSF7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7." class="big_lightbox"> HAF005). A specific band was detected for CD27/TNFRSF7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7." alt="Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 2 µg/mL of Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB574) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD27/TNFRSF7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF017). A specific band was detected for CD27/TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for CD27/TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lymph node tissue and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD27/TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

F0113) and Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." class="big_lightbox"> F0113) and Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." alt="Mouse splenocytes were labeled with Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB5741) followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113) and Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />