CD27/TNFRSF7 Antibody (57703)

In the presence of sub-optimal amounts of Mouse CD3 epsilon Monoclonal Anti­body (Catalog # MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recombinant Human CD27 Fc Chimera (Catalog # 382-CD) inhibits proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, inhibition of proliferation elicited by Recombinant Human CD27 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD27 Monoclonal Antibody (Catalog # MAB382). The ND50 is typically 0.2‑0.4 µg/mL.

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clone: 57703
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD27/TNFRSF7 Antibody (57703) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27
  Thr21-Ile192
  Accession # P26842
• Specificity: Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta , rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, or rhTNF RI is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD27
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.2-0.4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD27/TNFRSF7 Antibody (57703)

• CD27 antigen
• CD27 molecule
• CD27
• MGC20393
• S152CD27L receptor
• T cell activation antigen CD27
• T cell activation antigen S152
• T14
• TNFRSF7
• TNFRSF7T-cell activation antigen CD27
• Tp55
• Tumor necrosis factor receptor superfamily member 7
• tumor necrosis factor receptor superfamily, member 7

Background

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4⁺ compartment, it is preferentially expressed on CD45RA⁺ cells. In contrast, it is preferentially expressed on CD45RO⁺ cells in the CD8⁺ compartment. CD27 also appears to be a potential marker for memory B cells. It exists as both a disulfide‑linked dimer on the cell surface and as a soluble protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 20 aa signal, a 173 aa extracellular domain, a 20 aa transmembrane domain, and a 47 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and a small subset of activated T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses.

1. Camerini, D. et al. (1991) J. Immunol. 147:3165.
2. Loenen, W.A. et al. (1992) J. Immunol. 149:3937.
3. Lens, S.M.A. et al. (1998) Sem. Immunol. 10:491.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD27/TNFRSF7 Antibody (MAB382)(4)

Publications using MAB382

• Van den Eynde, A;Gehrcken, L;Verhezen, T;Lau, HW;Hermans, C;Lambrechts, H;Flieswasser, T;Quatannens, D;Roex, G;Zwaenepoel, K;Marcq, E;Joye, P;Cardenas De La Hoz, E;Deben, C;Gasparini, A;Montay-Gruel, P;Le Compte, M;Lion, E;Lardon, F;Van Laere, S;Siozopoulou, V;Campillo-Davo, D;De Waele, J;Pauwels, P;Jacobs, J;Smits, E;Van Audenaerde, JRM; IL-15-secreting CAR natural killer cells directed toward the pan-cancer target CD70 eliminate both cancer cells and cancer-associated fibroblasts Journal of hematology & oncology 2024-02-09 [PMID: 38331849] (Neutralization, Human)
• Yoshiaki Abe, Mamiko Sakata-Yanagimoto, Manabu Fujisawa, Hiroaki Miyoshi, Yasuhito Suehara, Keiichiro Hattori et al. A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling Nature Cell Biology 2022-04-01 [PMID: 35332263]
• M Boccazzi, J Van Steenw, AL Schang, V Faivre, T Le Charpen, C Bokobza, Z Csaba, C Verderio, M Fumagalli, S Mani, P Gressens The immune-inflammatory response of oligodendrocytes in a murine model of preterm white matter injury: the role of TLR3 activation Cell Death & Disease, 2021-02-08;12(2):166. 2/8/2021 [PMID: 33558485]
• Vranjkovic A, Crawley AM, Gee K, Kumar A, Angel JB IL-7 decreases IL-7 receptor alpha (CD127) expression and induces the shedding of CD127 by human CD8+ T cells. Int. Immunol., 2007-10-22;19(12):1329-39. 2007-10-22 [PMID: 17956896] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD27
• Uniprot:
  • P26842()