Human CD25/IL-2R alpha Recombinant Protein

Product Discontinued

Human CD25/IL-2R alpha Recombinant Protein Summary

• Description: A single glycosylated polypeptide protein corresponding to amino acids Glu22 - Cys213 of IL2RA (P01589).
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Lyophilized from a 0.2 um filtered concentrated solution in PBS.
• Details of Functionality: The ED50, as determined by the inhibition of rh IL-2-dependent proliferation of MO7e cells, is less than 0.75 mg/mL.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: IL2RA
• Endotoxin Note: Less than 1 EU/ug of rHuIL-2 R alpha as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with sterilized PBS to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human CD25/IL-2R alpha Recombinant Protein

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

CD25, also known as IL-2 R alpha, is a 55 kDa transmembrane protein in the common gamma chain (gamma c) family of cytokine receptors. It is primarily expressed on activated T cells and on regulatory T cells (Treg). A soluble form of CD25 can be generated by proteolytic cleavage of the cell surface receptor. CD25 associates with IL-2 R beta and gamma c/IL-2 R gamma/CD132 to form a high affinity receptor complex for IL-2. CD25 is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones. It is also required for the development of CD4+CD25+ Treg cells which suppress autoreactive CD4+ T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL2RA