Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

ELISA: CD25/IL-2 R alpha ELISA Kit [NBP1-88106] - Standard curve

Product Discontinued

Human CD25/IL-2R alpha ELISA Kit (Colorimetric) Summary

• Description: The human sIL-2R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sIL-2R
• Specificity: This assay detects soluble IL-2R in human serum, plasma, buffered solution, or cell culture medium. It will exclusively recognize both natural and recombinant human IL-2R.
• Standard Curve Range: 0.31 - 20.0 ng/mL
• Sensitivity: 0.27 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.8%
• Intra-Assay: CV% < 7.2%
• Spike Recovery: 75%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL2RA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 has been described as a factor that promotes the growth and proliferation of human T-cells thus occupying a pivotal role in the generation of the immune response. This proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific cell surface receptor following T lymphocyte activation. The receptor for IL-2 is composed of at least three distinct polypeptide subunits , the IL-2R-alpha, IL-2R-beta, and the IL-2R-gamma chains giving rise to a 55-65 kD membrane bound protein. In addition to the de-novo expression of IL-2R by activated peripheral blood T-cells, a released and fully soluble form of IL-2R (sIL-2R) has been detected. It was shown that sIL-2R is present in vivo, at low levels in the sera of healthy persons and at markedly elevated levels in various pathological conditions like neoplastic disorders. Soluble IL-2R levels are significantly elevated in the sera of patients with adult T-cell leukemia, hairy cell leukemia, lymphocytic leukemia, Hodgkin's and Non-Hodgkin's lymphoma, liver, breast and lung cancer. In patients with systemic lupus erythematosus, rheumatoid arthritis, progressive systemic sclerosis, polymyositis, Kawasaki disease, multiple sclerosis, and Diabetes type I plasma concentrations of sIL-2 receptor are significantly elevated as compared to controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CD25/IL-2R alpha ELISA Kit (NBP1-88106). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL2RA