Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

ELISA: CD25/IL-2 R alpha ELISA Kit [NBP1-88106] - Standard curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
• Standard Curve Range: 0.31 - 20.0 ng/mL
• Sensitivity: 0.27 ng/mL

Human CD25/IL-2R alpha ELISA Kit (Colorimetric) Summary

• Description: The human sIL-2R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sIL-2R
• Specificity: This assay detects soluble IL-2R in human serum, plasma, buffered solution, or cell culture medium. It will exclusively recognize both natural and recombinant human IL-2R.
• Standard Curve Range: 0.31 - 20.0 ng/mL
• Sensitivity: 0.27 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.8%
• Intra-Assay: CV% < 7.2%
• Spike Recovery: 75%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL2RA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 has been described as a factor that promotes the growth and proliferation of human T-cells thus occupying a pivotal role in the generation of the immune response. This proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific cell surface receptor following T lymphocyte activation. The receptor for IL-2 is composed of at least three distinct polypeptide subunits , the IL-2R-alpha, IL-2R-beta, and the IL-2R-gamma chains giving rise to a 55-65 kD membrane bound protein. In addition to the de-novo expression of IL-2R by activated peripheral blood T-cells, a released and fully soluble form of IL-2R (sIL-2R) has been detected. It was shown that sIL-2R is present in vivo, at low levels in the sera of healthy persons and at markedly elevated levels in various pathological conditions like neoplastic disorders. Soluble IL-2R levels are significantly elevated in the sera of patients with adult T-cell leukemia, hairy cell leukemia, lymphocytic leukemia, Hodgkin's and Non-Hodgkin's lymphoma, liver, breast and lung cancer. In patients with systemic lupus erythematosus, rheumatoid arthritis, progressive systemic sclerosis, polymyositis, Kawasaki disease, multiple sclerosis, and Diabetes type I plasma concentrations of sIL-2 receptor are significantly elevated as compared to controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL2RA