CD25/IL-2R alpha Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CD25/IL-2R alpha Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
  Glu22-Lys236
  Accession # Q544I2
• Specificity: Detects mouse CD25/IL-2 R alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) CD25/IL-2 R alpha is observed. In Western blots, approximately 5% cross-reactivity with rhCD25/IL-2 R alpha is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-2 R beta and rmIL-2 R gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL2RA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture:Mouse CD25/IL-2 R alpha Antibody (Catalog # AF2438)
  ELISA Detection: Mouse CD25/IL-2 R alpha Biotinylated Antibody (Catalog # BAF2438)
  Standard: Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody [Biotin]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha , respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma _c. IL-2 R beta (CD122) and gamma _c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma _c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (12-14).

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
4. Miller, J. et al. (1985) J. Immunol. 134:4212.
5. Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
6. Hatakeyama, M. et al. (1989) Science 244:551.
7. Takeshita, T. et al. (1992) Science 257:379.
8. Wang, X. et al. (2005) Science 310:1159.
9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
11. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
12. Willerford, D.M. et al. (1995) Immunity 3:521.
13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (BAF2438)(2)

Publications using BAF2438

• Kim, SHJ;Lee, H;Gingras, A;Ley, K;Spangler, JB;Ginsberg, MH; A CD25-CCR7 complex initiates non-canonical IL-2 signaling bioRxiv : the preprint server for biology 2025-02-04 [PMID: 39975281] (Immunoprecipitation, Mouse)
• Sun, H;Lee, HS;Kim, SH;Fernandes de Lima, M;Gingras, AR;Du, Q;McLaughlin, W;Ablack, J;Lopez-Ramirez, MA;Lagarrigue, F;Fan, Z;Chang, JT;VanDyke, D;Spangler, JB;Ginsberg, MH; IL-2 can signal via chemokine receptors to promote regulatory T cells' suppressive function Cell reports 2023-08-29 [PMID: 37598341] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • Q544I2(Mouse)