CD25/IL-2R alpha Antibody

Canine peripheral blood monocytes were stained with Sheep Anti-Canine CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6227) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Allophycocyanin-conjugated Anti-Canine CD4. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

• Reactivity: Ca
• Applications: Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD25/IL-2R alpha Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant canine CD25/IL-2 R alpha
  Tyr19-Ile238
  Accession # BAI49682
• Specificity: Detects canine CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human CD25/IL-2 R alpha , recombinant mouse CD25/IL-2 R alpha , and recombinant rat CD25/IL-2 R alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IL2RA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c ). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 20 aa signal peptide, a 217 aa extracellular domain (ECD) with two Sushi domains, a 19 aa transmembrane segment, and an 12 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49% - 60% aa sequence identity with human, mouse, and rat IL-2 R alpha . IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4 + CD25 + Treg which suppress autoreactive CD4 + T cells, thereby contributing to peripheral T cell homeostasis (11-13).

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67.
3. Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638.
4. Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137.
5. Hatakeyama, M. et al. (1989) Science 244:551.
6. Takeshita, T. et al. (1992) Science 257:379.
7. Wang, X. et al. (2005) Science 310:1159.
8. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
9. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
11. Willerford, D.M. et al. (1995) Immunity 3:521.
12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • BAI49682()