CD25/IL-2R alpha Antibody (PC61) [Alexa Fluor® 488]

Product Discontinued

CD25/IL-2R alpha Antibody (PC61) [Alexa Fluor® 488] Summary

• Immunogen: The B6.1 mouse cytotoxic T cell line was used as immunogen for this antibody.
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2RA
• Purity: Protein G purified
• Endotoxin Note: < 0.06 EU/ug

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry
  • In vivo assay
• Application Notes: Mouse splenocytes were stimulated with 5 ug/ml ConA for three days prior to testing.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD25/IL-2R alpha Antibody (PC61) [Alexa Fluor® 488]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (NBP2-27425AF488)(3)

Publications using NBP2-27425AF488

• Salman S, Meyers DJ, Wicks EE Et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 2022-05-02 [PMID: 35499076] (FLOW, FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (FLOW, Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018-01-24 [PMID: 29367423] (FLOW, Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 form of this antbody

Review for CD25/IL-2R alpha Antibody (NBP2-27425AF488) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 05/03/2019

Summary

• Application: Flow Cytometry
• Sample Tested: Breast cancer tissue
• Species: Mouse
• Lot: 5924-01-NBP2

Bioinformatics

• Gene Symbol: IL2RA