CD25/IL-2R alpha Antibody (Hu107) [Unconjugated]

In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Human Anti-Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926). Human IgG Isotype Control (Catalog # 1-001-A) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).

Human PBMCs were treated with PHA (5 µg/mL for 48hr) then stained with (A) Human CD25/IL-2 R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: Hu107
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

CD25/IL-2R alpha Antibody (Hu107) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody For Research Use Only
• Immunogen: Human CD25/IL-2 R alpha
• Specificity: Detects Human CD25/IL-2 R alpha based on Basiliximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Basiliximab. This product is for research use only.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 0.5 ug/10^6 cells
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (Hu107) [Unconjugated]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

Basiliximab is a biosimilar directed against the extracellular domain of CD25/IL-2 R alpha . This product an be used in flow cytometry or various assay format to measure CD25/IL-2R alpha expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.