CD25/IL-2R alpha Antibody (Basiliximab) [Janelia Fluor® 549] - Chimeric


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Product Details

Reactivity Hu, Pm-Cm, RMSpecies Glossary
Applications WB, Flow, IHC, ICC/IF
Janelia Fluor 549

Order Details

CD25/IL-2R alpha Antibody (Basiliximab) [Janelia Fluor® 549] - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody.
Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit -binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ((alpha)(beta)(gamma)c) -T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
IgG Kappa
Protein A purified
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  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD25/IL-2R alpha Antibody (Basiliximab) [Janelia Fluor® 549] - Chimeric

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52661AF350
Alexa Fluor 405 NBP2-52661AF405
Alexa Fluor 488 NBP2-52661AF488
Alexa Fluor 532 NBP2-52661AF532
Alexa Fluor 594 NBP2-52661AF594
Alexa Fluor 647 NBP2-52661AF647
Alexa Fluor 700 NBP2-52661AF700
Alexa Fluor 750 NBP2-52661AF750
Allophycocyanin NBP2-52661APC
Biotin NBP2-52661B
DyLight 350 NBP2-52661UV
DyLight 405 NBP2-52661V
DyLight 488 NBP2-52661G
DyLight 550 NBP2-52661R
DyLight 650 NBP2-52661C
DyLight 680 NBP2-52661FR
DyLight 755 NBP2-52661IR
FITC NBP2-52661F
HRP NBP2-52661H
Janelia Fluor 549 NBP2-52661JF549
Janelia Fluor 646 NBP2-52661JF646
PE NBP2-52661PE
PE/Atto594 NBP2-52661PEATT594
PE/Cy5.5 NBP2-52661PECY55
PE/Cy7 NBP2-52661PECY7
PerCP NBP2-52661PCP

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Gene Symbol IL2RA