CD25/IL-2R alpha Antibody (280406) [Allophycocyanin]

CD4+mouse T cells treated with anti-mouse CD3 and anti-mouse CD28 for 24 hours were stained with Rat Anti-Mouse CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB2438A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 280406
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

CD25/IL-2R alpha Antibody (280406) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
  Glu22-Lys236
  Accession # P01590
• Specificity: Detects mouse CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) gamma _c, recombinant human CD25/IL-2 R alpha , rmIL-2 R beta , or rmIL-15 R is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (280406) [Allophycocyanin]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha , respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma _c. IL-2 R beta (CD122) and gamma _c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma _c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for Activation Induced Cell Death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (12‑14).

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
4. Miller, J. et al. (1985) J. Immunol. 134:4212.
5. Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
6. Hatakeyama, M. et al. (1989) Science 244:551.
7. Takeshita, T. et al. (1992) Science 257:379.
8. Wang, X. et al. (2005) Science 310:1159.
9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
11. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
12. Willerford, D.M. et al. (1995) Immunity 3:521.
13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (FAB2438A)(2)

Publications using FAB2438A

• H Zhang, S Ge, B Ni, K He, P Zhu, X Wu, Y Shao Augmenting ATG14 alleviates atherosclerosis and inhibits inflammation via promotion of autophagosome-lysosome fusion in macrophages Autophagy, 2021-05-04;0(0):. 2021-05-04 [PMID: 33849389] (Flow Cytometry, Mouse)
• Llopiz D, Dotor J, Casares N, Bezunartea J, Diaz-Valdes N, Ruiz M, Aranda F, Berraondo P, Prieto J, Lasarte JJ, Borras-Cuesta F, Sarobe P Peptide inhibitors of transforming growth factor-beta enhance the efficacy of antitumor immunotherapy. Int. J. Cancer, 2009-12-01;125(11):2614-23. 2009-12-01 [PMID: 19530254] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • P01590()