CD25/IL-2R alpha Antibody (24204) [Unconjugated]

Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.

CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB623) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

UBTD1 depletion slows down RTK degradation.(A–E) DU145 cells were transfected for 48 hr with the indicated siRNA (control, siCTRLpool or UBTD1: siUBTD1pool). (A) HGF-alexa647 pulse chase images and quantification. (B) Proximal ligation assay monitoring and quantification of MET associated with ubiquitin in DU145 treated with HGF (40 ng/ml, 10 min). Nuclei were stained with DAPI (blue) on the MERGE image. (C) Immunoblots show MET ubiquitylation in presence of HGF (40 ng/ml, 10 min) in HEK cells in different experimental conditions. Cells were transfected, as indicated, with expression vectors for histidine-tagged ubiquitin (His-Ub) and MET. His-Ub crosslinked forms of MET were purified (IP: His) and the immunoblot of MET showed MET ubiquitylation. The immunoblot of MET (bottom panel) was performed in parallel to verify the amounts of MET protein engaged in His-Ub purifications. The immunoblot of UBTD1 shows the level of siRNA depletion. (D) Immunoblot and quantification of receptors. The immunoblot of UBTD1 shows the level of siRNA depletion. (E) mRNA quantification of receptors. mRNA of UBTD1 shows the level of siRNA depletion. Scale bar = 10 µm. n ≥ 3 independent experiments. ns = non-significant; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; (A,B) Bonferroni’s multiple comparison test; (D,E) two-way ANOVA and Bonferroni’s multiple comparisons test; data are mean ± s.e.m. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33884955), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, ELISA(Cap), ICC/IF
• Clone: 24204
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD25/IL-2R alpha Antibody (24204) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
  Glu22-Cys213
  Accession # P01589
• Specificity: Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha . In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta , rhIL-3 R alpha , rhIL-4 R, rhIL-5 R alpha , rhIL-5 R beta , rhIL-6 R, or rhgp130.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RA
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CD25/IL-2 R alpha Biotinylated Antibody (Catalog number BAF223)
  Standard: Recombinant Human CD25/IL-2 R alpha (Catalog number 223-2A)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (24204) [Unconjugated]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (MAB623)(6)

Publications using MAB623

• Mo, Y;Yue, M;Yim, LY;Zhou, R;Yu, C;Peng, Q;Zhou, Y;Luk, TY;Lui, GC;Huang, H;Lim, CYH;Wang, H;Liu, L;Sun, H;Wang, J;Song, Y;Chen, Z; Nicotinamide mononucleotide impacts HIV-1 infection by modulating immune activation in T lymphocytes and humanized mice EBioMedicine 2023-11-17 [PMID: 37980794] (IHC, Xenograft)
• Torrino S, Tiroille V, Dolfi B Et al. UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling eLife 4/22/2021 [PMID: 33884955]
• Charles J.M. Bell, Yongliang Sun, Urszula M. Nowak, Jan Clark, Sarah Howlett, Marcin L. Pekalski et al. Sustained in vivo signaling by long-lived IL-2 induces prolonged increases of regulatory T cells Journal of Autoimmunity 2015-01-01 [PMID: 25457307] (ELISA Capture, Cynomolgus Monkey)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)
• Akoum A, Al-Akoum M, Lemay A, Maheux R, Leboeuf M Imbalance in the peritoneal levels of interleukin 1 and its decoy inhibitory receptor type II in endometriosis women with infertility and pelvic pain. Fertil. Steril., 2007-10-24;89(6):1618-24. 2007-10-24 [PMID: 17919610] (ELISA Development, Human)
• Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Biochem. J., 2004-06-01;380(0):425-33. 2004-06-01 [PMID: 14992689] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • P01589()