CD25/IL-2R alpha Antibody (22722) [Unconjugated]

CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB223). The ND50 is typically 0.5‑1.0 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ICC/IF
• Clone: 22722
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD25/IL-2R alpha Antibody (22722) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
  Glu22-Cys213
  Accession # P01589
• Specificity: Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma , rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha , rhIL-6 R, rhIL‑7 R, rhIL-9 R, and rhIL-10 R is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RA
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.5-1.0 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (22722) [Unconjugated]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (MAB223)(9)

Publications using MAB223

• Sigutova, V;Xiang, W;Regensburger, M;Winner, B;Prots, I; Alpha-synuclein fine-tunes neuronal response to pro-inflammatory cytokines Brain, behavior, and immunity 2024-08-14 [PMID: 39128571] (Bioassay, Human)
• Wu, WC;Shiu, C;Tong, TK;Leung, SO;Hui, CW; Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases Journal of immunology research 2023-12-08 [PMID: 38106519] (Neutralization, Human)
• S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human)
• Thai H. Ho, Kirsten Pfeffer, Glen J. Weiss, Yvette Ruiz, Douglas F. Lake Identification of a CD4+ T cell line with Treg-like activity Human Immunology 2022-04-01 [PMID: 35094878]
• Alireza Faridar, Aaron D Thome, Weihua Zhao, Jason R Thonhoff, David R Beers, Belen Pascual et al. Restoring regulatory T-cell dysfunction in Alzheimer’s disease through ex vivo expansion Brain Communications 2020-07-20 [PMID: 32954348]
• F Porichis, MG Hart, A Massa, HL Everett, A Morou, J Richard, N Brassard, M Veillette, M Hassan, NL Ly, JP Routy, GJ Freeman, M Dubé, A Finzi, DE Kaufmann Immune Checkpoint Blockade Restores HIV-Specific CD4 T Cell Help for NK Cells J. Immunol., 2018-06-22;0(0):. 2018-06-22 [PMID: 29934472] (Neutralization, Human)
• Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016-09-02;6(0):32320. 2016-09-02 [PMID: 27586092] (Flow Cytometry, Neutralization, Human)
• Rebecca G. Oas, Benjamin A. Nanes, Chimdimnma C. Esimai, Peter A. Vincent, Andrés J. García, Andrew P. Kowalczyk p120-catenin and beta -catenin differentially regulate cadherin adhesive function Molecular Biology of the Cell 2013-03-15 [PMID: 23325790]
• Bhatt S, Zalcman S, Hassanain M, Siegel A Cytokine modulation of defensive rage behavior in the cat: role of GABAA and interleukin-2 receptors in the medial hypothalamus. Neuroscience, 2005-01-01;133(1):17-28. 2005-01-01 [PMID: 15893628] (Neutralization, Feline)

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • P01589()