CD200/OX2 Antibody [Unconjugated]


Western blot shows lysates of human, mouse, and rat brain (cortex) tissue, human tonsil tissue, and human dendritic cells (mature). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD200 more
CD200 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) at 15 µg/mL overnight at 4 °C. more
Simple Western lane view shows lysates of human, mouse, and rat brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for CD200 at approximately 70-75 kDa (as indicated) using 10 µg/mL of Goat more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready

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CD200/OX2 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human CD200
Accession # P41217
Detects human CD200 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Read Publications using
AF2724 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD200/OX2 Antibody [Unconjugated]

  • antigen identified by monoclonal MRC OX-2
  • CD200 antigenMOX1
  • CD200 molecule
  • CD200
  • MOX1
  • MOX2
  • MRC OX-2 antigen
  • MRC
  • OX-2 membrane glycoprotein
  • OX-2


CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5-7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact (6, 7, 10). In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14-16).

  1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  3. McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
  4. Wright, G.J. et al. (2001) Immunology 102:173.
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  8. Hoek, R.M. et al. (2000) Science 290:1768.
  9. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  10. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  11. Borriello, F. et al. (1997) J. Immunol. 158:4548.
  12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  13. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  14. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
  15. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
  16. Langlais, C.L. et al. (2006) J. Virol. 80:3098.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD200/OX2 Antibody (AF2724)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 10.
Publications using AF2724 Applications Species
X Zhang, W Jiang, C Xie, X Wu, Q Ren, F Wang, X Shen, Y Hong, H Wu, Y Liao, Y Zhang, R Liang, W Sun, Y Gu, T Zhang, Y Chen, W Wei, S Zhang, W Zou, H Ouyang Msx1+ stem cells recruited by bioactive tissue engineering graft for bone regeneration Nature Communications, 2022;13(1):5211. 2022 [PMID: 36064711] (Flow Cytometry, Rat) Flow Cytometry Rat
DT Farmer, H Mlcochova, Y Zhou, N Koelling, G Wang, N Ashley, H Bugacov, HJ Chen, R Parvez, KC Tseng, AE Merrill, RE Maxson, AOM Wilkie, JG Crump, SRF Twigg The developing mouse coronal suture at single-cell resolution Nature Communications, 2021;12(1):4797. 2021 [PMID: 34376651] (IHC, Mouse) IHC Mouse
N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020;11(1):5171. 2020 [PMID: 33057002] (IHC, Mouse) IHC Mouse
P Hingorani, V Dinu, X Zhang, H Lei, JF Shern, J Park, J Steel, F Rauf, D Parham, J Gastier-Fo, D Hall, DS Hawkins, SX Skapek, J Labaer, TA McEachron Transcriptome analysis of desmoplastic small round cell tumors identifies actionable therapeutic targets: a report from the Children's Oncology Group Sci Rep, 2020;10(1):12318. 2020 [PMID: 32703985] (Western Blot, Human) Western Blot Human
M Ishibashi, S Neri, H Hashimoto, T Miyashita, T Yoshida, Y Nakamura, H Udagawa, K Kirita, S Matsumoto, S Umemura, K Yoh, S Niho, M Tsuboi, K Masutomi, K Goto, A Ochiai, G Ishii CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors Sci Rep, 2017;7(0):46662. 2017 [PMID: 28429795] (IHC, Human) IHC Human
Douglas G Walker, Lih-Fen Lue, Tiffany M Tang, Charles H Adler, John N Caviness, Marwan N Sabbagh, Geidy E Serrano, Lucia I Sue, Thomas G Beach Changes in CD200 and intercellular adhesion molecule-1 (ICAM-1) levels in brains of Lewy body disorder cases are associated with amounts of Alzheimer's pathology not ?-synuclein pathology. Neurobiology of Aging, 2017;0(0):1558-1497. 2017 [PMID: 28390825] (Western Blot, Human) Western Blot Human
Dorfman , David M, Shahsafaei , Aliakbar, Alonso , Miguel A Utility of CD200 immunostaining in the diagnosis of primary mediastinal large B cell lymphoma: comparison with MAL, CD23, and other markers. Mod Pathol, 2012;25(12):1637-43. 2012 [PMID: 22899296] (IHC-P, Human) IHC-P Human
Kelly OG, Chan MY, Martinson LA Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat. Biotechnol., 2011;29(8):750-6. 2011 [PMID: 21804561] (IHC-Fr, Human) IHC-Fr Human
Garza LA, Yang CC, Zhao T, Blatt HB, Lee M, He H, Stanton DC, Carrasco L, Spiegel JH, Tobias JW, Cotsarelis G Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells. J. Clin. Invest., 2011;121(2):613-22. 2011 [PMID: 21206086] (IHC-P, Human) IHC-P Human
Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, Thomas NE, Serody JS, Sharpless NE CD200 is induced by ERK and is a potential therapeutic target in melanoma. J. Clin. Invest., 2007;117(12):3922-3929. 2007 [PMID: 18008004] (ICC, Human) ICC Human

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Gene Symbol CD200