CD200/OX2 Antibody [Unconjugated]

Western blot shows lysates of human, mouse, and rat brain (cortex) tissue, human tonsil tissue, and human dendritic cells (mature). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD200 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD200 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human, mouse, and rat brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for CD200 at approximately 70-75 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD200/OX2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD200
  Gln31-Gly232
  Accession # P41217
• Specificity: Detects human CD200 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD200
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD200/OX2 Antibody [Unconjugated]

• antigen identified by monoclonal MRC OX-2
• CD200 antigenMOX1
• CD200 molecule
• CD200
• MOX1
• MOX2
• MOX2MRC
• MRC OX-2 antigen
• MRC
• OX-2 membrane glycoprotein
• OX-2

Background

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5-7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact (6, 7, 10). In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14-16).

1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
3. McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
4. Wright, G.J. et al. (2001) Immunology 102:173.
5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
8. Hoek, R.M. et al. (2000) Science 290:1768.
9. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
10. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
11. Borriello, F. et al. (1997) J. Immunol. 158:4548.
12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
13. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
14. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
15. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
16. Langlais, C.L. et al. (2006) J. Virol. 80:3098.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD200/OX2 Antibody (AF2724)(10)

Publications using AF2724

• X Zhang, W Jiang, C Xie, X Wu, Q Ren, F Wang, X Shen, Y Hong, H Wu, Y Liao, Y Zhang, R Liang, W Sun, Y Gu, T Zhang, Y Chen, W Wei, S Zhang, W Zou, H Ouyang Msx1+ stem cells recruited by bioactive tissue engineering graft for bone regeneration Nature Communications, 2022;13(1):5211. 2022 [PMID: 36064711] (Flow Cytometry, Rat)
• DT Farmer, H Mlcochova, Y Zhou, N Koelling, G Wang, N Ashley, H Bugacov, HJ Chen, R Parvez, KC Tseng, AE Merrill, RE Maxson, AOM Wilkie, JG Crump, SRF Twigg The developing mouse coronal suture at single-cell resolution Nature Communications, 2021;12(1):4797. 2021 [PMID: 34376651] (IHC, Mouse)
• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020;11(1):5171. 2020 [PMID: 33057002] (IHC, Mouse)
• P Hingorani, V Dinu, X Zhang, H Lei, JF Shern, J Park, J Steel, F Rauf, D Parham, J Gastier-Fo, D Hall, DS Hawkins, SX Skapek, J Labaer, TA McEachron Transcriptome analysis of desmoplastic small round cell tumors identifies actionable therapeutic targets: a report from the Children's Oncology Group Sci Rep, 2020;10(1):12318. 2020 [PMID: 32703985] (Western Blot, Human)
• M Ishibashi, S Neri, H Hashimoto, T Miyashita, T Yoshida, Y Nakamura, H Udagawa, K Kirita, S Matsumoto, S Umemura, K Yoh, S Niho, M Tsuboi, K Masutomi, K Goto, A Ochiai, G Ishii CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors Sci Rep, 2017;7(0):46662. 2017 [PMID: 28429795] (IHC, Human)
• Douglas G Walker, Lih-Fen Lue, Tiffany M Tang, Charles H Adler, John N Caviness, Marwan N Sabbagh, Geidy E Serrano, Lucia I Sue, Thomas G Beach Changes in CD200 and intercellular adhesion molecule-1 (ICAM-1) levels in brains of Lewy body disorder cases are associated with amounts of Alzheimer's pathology not ?-synuclein pathology. Neurobiology of Aging, 2017;0(0):1558-1497. 2017 [PMID: 28390825] (Western Blot, Human)
• Dorfman , David M, Shahsafaei , Aliakbar, Alonso , Miguel A Utility of CD200 immunostaining in the diagnosis of primary mediastinal large B cell lymphoma: comparison with MAL, CD23, and other markers. Mod Pathol, 2012;25(12):1637-43. 2012 [PMID: 22899296] (IHC-P, Human)
• Kelly OG, Chan MY, Martinson LA Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat. Biotechnol., 2011;29(8):750-6. 2011 [PMID: 21804561] (IHC-Fr, Human)
• Garza LA, Yang CC, Zhao T, Blatt HB, Lee M, He H, Stanton DC, Carrasco L, Spiegel JH, Tobias JW, Cotsarelis G Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells. J. Clin. Invest., 2011;121(2):613-22. 2011 [PMID: 21206086] (IHC-P, Human)
• Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, Thomas NE, Serody JS, Sharpless NE CD200 is induced by ERK and is a potential therapeutic target in melanoma. J. Clin. Invest., 2007;117(12):3922-3929. 2007 [PMID: 18008004] (ICC, Human)

Bioinformatics

• Gene Symbol: CD200
• Uniprot:
  • P41217()