HAF017). A specific band was detected for CD200 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human, mouse, and rat brain (cortex) tissue, human tonsil tissue, and human dendritic cells (mature). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD200 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2724-SP | |||
HAF019). A specific band was detected for CD200 at approximately 38-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3355) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD200 at approximately 38-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3355-SP | |||
2724-CD) was serially diluted and captured by Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CD200 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) was serially diluted and captured by Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CD200 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB27243-SP | |||
| Carrier Free | MAB27243-500 | |||
3414-CD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD200 His-tag Avi-tag (Catalog # AVI10032) binds with an ED50 of 0.1-0.6 µg/mL. " alt="When Recombinant Human CD200R1 Fc Chimera (3414-CD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD200 His-tag Avi-tag (Catalog # AVI10032) binds with an ED50 of 0.1-0.6 µg/mL. " class="big_thumb" />
FAB4867P) and either (A) Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27241) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27241) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />
2554-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Mouse CD200 (Catalog # 9867-CD) binds with an ED50 of 0.25-1.5 µg/mL." alt="When Recombinant Mouse CD200 R1 Fc Chimera Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Mouse CD200 (Catalog # 9867-CD) binds with an ED50 of 0.25-1.5 µg/mL." class="big_thumb" />
3414-CD) is coated at 2 µg/mL, 100 µL/well, Recombinant Cynomolgus Monkey CD200 (Catalog # 10086-CD) binds with an ED50 of 0.04-0.24 µg/mL." alt="When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is coated at 2 µg/mL, 100 µL/well, Recombinant Cynomolgus Monkey CD200 (Catalog # 10086-CD) binds with an ED50 of 0.04-0.24 µg/mL." class="big_thumb" />
3414-CD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD200 Fc Chimera Avi-tag (Catalog # AVI2724) binds with an ED50 of 2-10 ng/mL." alt="When Recombinant Human CD200R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD200 Fc Chimera Avi-tag (Catalog # AVI2724) binds with an ED50 of 2-10 ng/mL." class="big_thumb" />
3414-CD) is immobilized at 2 µg/mL, Recombinant Human CD200 (Catalog # 10032-CD) binds with an ED50 of 0.2-1.2 µg/mL." alt="When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 µg/mL, Recombinant Human CD200 (Catalog # 10032-CD) binds with an ED50 of 0.2-1.2 µg/mL." class="big_thumb" />
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes of hippocampal neurons. View our protocol for 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD200/OX2 with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32132, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414333642.jpg "Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD200/OX2 with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32132, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414304113.jpg)
![Flow Cytometry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32132, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-29202416453219.jpg)
![Western blot analysis on different lysates with NBP3-32132 at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: NCI-H226 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (30 µg/Lane) Lane 4: Human placenta tissue lysate (30 µg/Lane) Lane 5: Human lung tissue lysate (30 µg/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32132 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32132_-western-blot-639204595243814584.jpg)
