CD200/OX2 Antibodies: Novus Biologicals

AF2724

CD200/OX2 Antibody [Unconjugated]

100 μg

AF2724

HAF017). A specific band was detected for CD200 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human, mouse, and rat brain (cortex) tissue, human tonsil tissue, and human dendritic cells (mature). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for CD200 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human, mouse, and rat brain (cortex) tissue, human tonsil tissue, and human dendritic cells (mature). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD200 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD200 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human, mouse, and rat brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for CD200 at approximately 70-75 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2724) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

$Studies with the Kasumi-1 cell line and with exosomes derived from Kasumi-1 culture supernatants. A) Flow cytometry of Kasumi-1 cells shows expression of blast markers, CD34, CD117 and CD33 and of CD81 (tetraspanin). Dotted lines indicate isotype controls. B) Kasumi-1 exosomes were separated on a continuous sucrose density gradient, and the collected gradient fractions were tested in Western blots for CD34 and CD81. CD34+ and CD81+ exosomes were recovered at the density of 1.10–1.14. C) An electron microscope image of isolated and negatively stained Kasumi-1 exosomes. D) Western blots of isolated Kasumi-1 exosomes (Kas) and of exosomes isolated from plasma of a normal donor (NC). Each lane was loaded with 10 µg exosomal protein. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25093329), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2724U-100UG
Alexa Fluor 405	AF2724V-100UG
Alexa Fluor 488	AF2724G-100UG
Alexa Fluor 532	AF2724X-100UG
Alexa Fluor 594	AF2724T-100UG
Alexa Fluor 647	AF2724R-100UG
Alexa Fluor 700	AF2724N-100UG
Alexa Fluor 750	AF2724S-100UG
Alexa Fluor Plus 405	AF2724AFP405-100UG
Alexa Fluor Plus 488	AF2724AFP488-100UG
Alexa Fluor Plus 555	AF2724AFP555-100UG
Alexa Fluor Plus 594	AF2724AFP594-100UG
Alexa Fluor Plus 647	AF2724AFP647-100UG
Alexa Fluor Plus 680	AF2724AFP680-100UG
Allophycocyanin	AF2724A
Biotin	BAF2724
CoraFluor 1	AF2724CL1
DyLight 350	AF2724D
DyLight 405	AF2724E
DyLight 488	AF2724K
DyLight 550	AF2724L
DyLight 594	AF2724M
DyLight 650	AF2724W
DyLight 680	AF2724Y
DyLight 755	AF2724Z
FITC	AF2724F
HRP	AF2724H
Janelia Fluor 525	AF2724JF525
Janelia Fluor 549	AF2724I
Janelia Fluor 646	AF2724J
Janelia Fluor 669	AF2724JF669
PE	AF2724P
PerCP	AF2724C
mFluor Violet 450 SE	AF2724MFV450
mFluor Violet 500 SE	AF2724MFV500
mFluor Violet 610 SE	AF2724MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2724-SP

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AF3355

CD200/OX2 Antibody [Unconjugated]

(5 Publications)

100 μg

AF3355

HAF019). A specific band was detected for CD200 at approximately 38-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3355) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for CD200 at approximately 38-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3355) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD200 at approximately 38-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes of hippocampal neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

CD200 was detected in perfusion fixed frozen sections of normal mouse brain using Goat Anti-Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3355) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes of hippocampal neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse brain (cortex) tissue and rat brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for CD200 at approximately 73-76 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse/Rat CD200 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3355) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

<b>Genetic Strategies Validation. </b>Expression of CD200 protein isoforms in neocortex, hippocampus and striatum of HdhQ7/7 and HdhQ111/7 knock-in mice were analyzed at 6, 8 and 13 months of age by Western blot.CD200full and CD200tr isoforms are observed in the three brain regions. Quantification of CD200 total protein, both isoforms, have been performed (A) in neocortex at 6 (HdhQ7/7 = 13, HdhQ111/7 = 10), 8 (HdhQ7/7 = 7, HdhQ111/7 = 7) and 13 (HdhQ7/7 = 7, HdhQ111/7 = 7) months of age; (B) in hippocampus at 6 (HdhQ7/7 = 8, HdhQ111/7 = 13), 8 (HdhQ7/7 = 7, HdhQ111/7 = 7) and 13 (HdhQ7/7 = 7, HdhQ111/7 = 7) months of age; and (C) in striatum at 6 (HdhQ7/7 = 14, HdhQ111/7 = 10), 8 (HdhQ7/7 = 14, HdhQ111/7 = 10) and 13 (HdhQ7/7 = 7, HdhQ111/7 = 7) months of age. Bars represent the mean ± SEM. p-Values are the result of the multiple t-tests of the HdhQ111/7 genotype versus the respective HdhQ7/7 group. Representative Western blots for 8 months of age are shown as the percentage of immunosignal ratio between CD200 and beta -actin relative to respective HdhQ7/7 brain region. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31790427), licensed under a CC-BY license. Not internally tested by R&D Systems.

A beta modulates CD200R-associated signaling. A. Incubation of purified astrocytes in the presence of A beta decreased CD200 expression as illustrated in the sample immunoblot and in the densitometric data (**P < 0.01; Student’s t-test for independent means; n = 6). B and C. A beta significantly increased CD200R mRNA on purified microglia (B) and the percentage of CD200R+ CD11b+ cells (C; *P < 0.05; **P < 0.01; Student’s t-test for independent means; n = 6). D and E. Whereas A beta decreased Dok2 phosphorylation (D), CD200Fc increased its phosphorylation in a time-dependent manner (E). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22642833), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of CD200 protein isoforms in R6/1 mouse brains at 8, 12, 20, and 30 weeks of age.(A) Neocortex, hippocampus and striatum were analyzed by Western blot and CD200 protein levels detected using beta -actin as loading control. Both isoforms, CD200full and the truncated CD200tr, are observed in all brain regions under analysis. Quantification of total CD200 protein (both isoforms) was measured in control (wt) and R6/1 mice: (B) in neocortex at 8 (wt = 6; R6/1 = 6), 12 (wt = 3; R6/1 = 3), 20 (wt = 3; R6/1 = 3) and 30 (wt = 3; R6/1 = 3) weeks of age; (C) in hippocampus at 8 (wt = 6; R6/1 = 6), 12 (wt = 5; R6/1 = 5), 20 (wt = 3; R6/1 = 3) and 30 (wt = 6; R6/1 = 6) weeks of age; and (D) in striatum at 8 (wt = 4; R6/1 = 5), 12 (wt = 3; R6/1 = 3), 20 (wt = 3; R6/1 = 3) and 30 (wt = 8; R6/1 = 8) weeks of age. Data are shown as percentage of immunosignal ratio between CD200 and beta -actin in R6/1 relative to their respective wild-type mice. Bars represent the means ± standard error of the means (SEM) of 3–8 animals. p-Values are the result of the multiple t-tests of the R6/1 genotype versus the respective wild-type group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31790427), licensed under a CC-BY license. Not internally tested by R&D Systems.

CD200R1 and CD200 protein levels in the post-mortem brain of Parkinson’s disease patients. Expression of CD200R1 protein isoforms 1 and 4 and CD200 proteins in the substantia nigra (SN) (a), frontal cortex (b), and hippocampus (c) tissue homogenates from control individuals (C, n = 4–8) and Parkinson’s disease patients (PD, n = 11–21) by western blot. Representative immunoblots are presented. Protein levels were normalized relative to beta tubulin. Data are expressed as fold change relative to C and are depicted as individual points with bars showing means ± SEM. *p < 0.05 and **p < 0.01 vs. C; Mann-Whitney test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35296683), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3355U-100UG
Alexa Fluor 405	AF3355V-100UG
Alexa Fluor 488	AF3355G-100UG
Alexa Fluor 532	AF3355X-100UG
Alexa Fluor 594	AF3355T-100UG
Alexa Fluor 647	AF3355R-100UG
Alexa Fluor 700	AF3355N-100UG
Alexa Fluor 750	AF3355S-100UG
Alexa Fluor Plus 405	AF3355AFP405-100UG
Alexa Fluor Plus 488	AF3355AFP488-100UG
Alexa Fluor Plus 555	AF3355AFP555-100UG
Alexa Fluor Plus 594	AF3355AFP594-100UG
Alexa Fluor Plus 647	AF3355AFP647-100UG
Alexa Fluor Plus 680	AF3355AFP680-100UG
Biotin	BAF3355
CoraFluor 1	AF3355CL1
DyLight 350	AF3355D
DyLight 405	AF3355E
DyLight 488	AF3355K
DyLight 550	AF3355L
DyLight 594	AF3355M
DyLight 650	AF3355W
DyLight 680	AF3355Y
DyLight 755	AF3355Z
FITC	AF3355F
HRP	AF3355H
Janelia Fluor 525	AF3355JF525
Janelia Fluor 549	AF3355I
Janelia Fluor 646	AF3355J
Janelia Fluor 669	AF3355JF669
PerCP	AF3355C
mFluor Violet 450 SE	AF3355MFV450
mFluor Violet 500 SE	AF3355MFV500
mFluor Violet 610 SE	AF3355MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3355-SP

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MAB27243

CD200/OX2 Antibody (325531) [Unconjugated]

(1 Publication)

100 μg

MAB27243

2724-CD) was serially diluted and captured by Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CD200 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox">

Recombinant Human CD200 Fc Chimera (Catalog # <a class=

2724-CD) was serially diluted and captured by Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CD200 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) was serially diluted and captured by Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CD200 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB27243U-100UG
Alexa Fluor 405	FAB27243V-100UG
Alexa Fluor 488	FAB27243G-100UG
Alexa Fluor 532	FAB27243X-100UG
Alexa Fluor 594	FAB27243T-100UG
Alexa Fluor 647	FAB27243R-100UG
Alexa Fluor 700	FAB27243N-100UG
Alexa Fluor 750	FAB27243S-100UG
Alexa Fluor Plus 405	FAB27243AFP405-100UG
Alexa Fluor Plus 488	FAB27243AFP488-100UG
Alexa Fluor Plus 555	FAB27243AFP555-100UG
Alexa Fluor Plus 594	FAB27243AFP594-100UG
Alexa Fluor Plus 647	FAB27243AFP647-100UG
Alexa Fluor Plus 680	FAB27243AFP680-100UG
Allophycocyanin	FAB27243A
Biotin	FAB27243B
CoraFluor 1	FAB27243CL1
DyLight 350	FAB27243D
DyLight 405	FAB27243E
DyLight 488	FAB27243K
DyLight 550	FAB27243L
DyLight 594	FAB27243M
DyLight 650	FAB27243W
DyLight 680	FAB27243Y
DyLight 755	FAB27243Z
FITC	FAB27243F
HRP	FAB27243H
Janelia Fluor 525	FAB27243JF525
Janelia Fluor 549	FAB27243I
Janelia Fluor 646	FAB27243J
Janelia Fluor 669	FAB27243JF669
PE	FAB27243P
PerCP	FAB27243C
mFluor Violet 450 SE	FAB27243MFV450
mFluor Violet 500 SE	FAB27243MFV500
mFluor Violet 610 SE	FAB27243MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB27243-SP
Carrier Free	MAB27243-500

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NBP3-32132

CD200/OX2 Antibody (PSH01-48)

100 ul

NBP3-32132

Flow Cytometry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32132, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western blot analysis on different lysates with NBP3-32132 at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: NCI-H226 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (30 µg/Lane) Lane 4: Human placenta tissue lysate (30 µg/Lane) Lane 5: Human lung tissue lysate (30 µg/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32132 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB27241

CD200/OX2 Antibody (325516) [Unconjugated]

100 μg

MAB27241

FAB4867P) and either (A) Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27241) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox">

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # <a class=

FAB4867P) and either (A) Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27241) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD200 Monoclonal Antibody (Catalog # MAB27241) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB27241U-100UG
Alexa Fluor 405	FAB27241V-100UG
Alexa Fluor 488	FAB27241G-100UG
Alexa Fluor 532	FAB27241X
Alexa Fluor 594	FAB27241T-100UG
Alexa Fluor 647	FAB27241R-100UG
Alexa Fluor 700	FAB27241N-100UG
Alexa Fluor 750	FAB27241S-100UG
Alexa Fluor Plus 405	FAB27241AFP405-100UG
Alexa Fluor Plus 488	FAB27241AFP488-100UG
Alexa Fluor Plus 555	FAB27241AFP555-100UG
Alexa Fluor Plus 594	FAB27241AFP594-100UG
Alexa Fluor Plus 647	FAB27241AFP647-100UG
Alexa Fluor Plus 680	FAB27241AFP680-100UG
Allophycocyanin	FAB27241A
Allophycocyanin/Cy7	FAB27241APCCY7
Biotin	FAB27241B
CoraFluor 1	FAB27241CL1
DyLight 350	FAB27241D
DyLight 405	FAB27241E
DyLight 488	FAB27241K
DyLight 550	FAB27241L
DyLight 594	FAB27241M
DyLight 650	FAB27241W
DyLight 680	FAB27241Y
DyLight 755	FAB27241Z
Fluorescein	FAB27241F
HRP	FAB27241H
Janelia Fluor 525	FAB27241JF525
Janelia Fluor 549	FAB27241I
Janelia Fluor 646	FAB27241J
Janelia Fluor 669	FAB27241JF669
PE/Atto594	FAB27241PEATT594
PE/Cy5.5	FAB27241PECY55
PE/Cy7	FAB27241PECY7
Phycoerythrin	FAB27241P
mFluor Violet 450 SE	FAB27241MFV450
mFluor Violet 500 SE	FAB27241MFV500
mFluor Violet 610 SE	FAB27241MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB27241F
Carrier Free	FAB27241A
Carrier Free	FAB27241P
Carrier Free	MAB27241-SP
Carrier Free	FAB27241V-100UG
Carrier Free	FAB27241U-100UG
Carrier Free	FAB27241T-100UG
Carrier Free	FAB27241S-100UG
Carrier Free	FAB27241R-100UG
Carrier Free	FAB27241G-100UG
Carrier Free	FAB27241N-100UG

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NBP3-32133

CD200/OX2 Antibody (PSH01-49)

100 ul

NBP3-32133

Western blot analysis on different lysates with NBP3-32133 at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: NCI-H226 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (20 µg/Lane) Lane 4: Human lung tissue lysate (20 µg/Lane) Predicted band size: 31 kDa Observed band size: 40-50 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32133 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB100-64860

CD200/OX2 Antibody (OX-104) - BSA Free

0.1 mg

NB100-64860

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64860AF350
Alexa Fluor 405	NB100-64860AF405
Alexa Fluor 488	NB100-64860AF488
Alexa Fluor 532	NB100-64860AF532
Alexa Fluor 594	NB100-64860AF594
Alexa Fluor 647	NB100-64860AF647
Alexa Fluor 700	NB100-64860AF700
Alexa Fluor 750	NB100-64860AF750
Alexa Fluor Plus 405	NB100-64860AFP405
Alexa Fluor Plus 488	NB100-64860AFP488
Alexa Fluor Plus 555	NB100-64860AFP555
Alexa Fluor Plus 594	NB100-64860AFP594
Alexa Fluor Plus 647	NB100-64860AFP647
Alexa Fluor Plus 680	NB100-64860AFP680
Allophycocyanin	NB100-64860APC
Biotin	NB100-64860B
CoraFluor 1	NB100-64860CL1
DyLight 350	NB100-64860UV
DyLight 405	NB100-64860V
DyLight 488	NB100-64860G
DyLight 550	NB100-64860R
DyLight 594	NB100-64860DL594
DyLight 650	NB100-64860C
DyLight 680	NB100-64860FR
DyLight 755	NB100-64860IR
FITC	NB100-64860F
HRP	NB100-64860H
Janelia Fluor 525	NB100-64860JF525
Janelia Fluor 549	NB100-64860JF549
Janelia Fluor 646	NB100-64860JF646
Janelia Fluor 669	NB100-64860JF669
PE	NB100-64860PE
PerCP	NB100-64860PCP
mFluor Violet 450 SE	NB100-64860MFV450
mFluor Violet 500 SE	NB100-64860MFV500
mFluor Violet 610 SE	NB100-64860MFV610

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NB100-65320

CD200/OX2 Antibody (OX-2) - BSA Free

(1 Publication)

250 μg

NB100-65320

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65320AF350
Alexa Fluor 405	NB100-65320AF405
Alexa Fluor 488	NB100-65320AF488
Alexa Fluor 532	NB100-65320AF532
Alexa Fluor 594	NB100-65320AF594
Alexa Fluor 647	NB100-65320AF647
Alexa Fluor 700	NB100-65320AF700
Alexa Fluor 750	NB100-65320AF750
Allophycocyanin	NB100-65320APC
Biotin	NB100-65320B
CoraFluor 1	NB100-65320CL1
DyLight 350	NB100-65320UV
DyLight 405	NB100-65320V
DyLight 488	NB100-65320G
DyLight 550	NB100-65320R
DyLight 594	NB100-65320DL594
DyLight 650	NB100-65320C
DyLight 680	NB100-65320FR
DyLight 755	NB100-65320IR
FITC	NB100-65320F
HRP	NB100-65320H
Janelia Fluor 525	NB100-65320JF525
Janelia Fluor 549	NB100-65320JF549
Janelia Fluor 646	NB100-65320JF646
Janelia Fluor 669	NB100-65320JF669
PE	NB100-65320PE
PerCP	NB100-65320PCP
mFluor Violet 450 SE	NB100-65320MFV450
mFluor Violet 500 SE	NB100-65320MFV500
mFluor Violet 610 SE	NB100-65320MFV610

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NBP2-52438

CD200/OX2 Antibody (6E8B11) - BSA Free

0.1 mg

NBP2-52438

ELISA: CD200/OX2 Antibody (6E8B11) [NBP2-52438] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52438-0.025mg

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NBP2-89696

CD200/OX2 Antibody (333) - Azide and BSA Free

100 ul

NBP2-89696

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89696AF350
Alexa Fluor 405	NBP2-89696AF405
Alexa Fluor 488	NBP2-89696AF488
Alexa Fluor 532	NBP2-89696AF532
Alexa Fluor 594	NBP2-89696AF594
Alexa Fluor 647	NBP2-89696AF647
Alexa Fluor 700	NBP2-89696AF700
Alexa Fluor 750	NBP2-89696AF750
Alexa Fluor Plus 405	NBP2-89696AFP405
Alexa Fluor Plus 488	NBP2-89696AFP488
Alexa Fluor Plus 555	NBP2-89696AFP555
Alexa Fluor Plus 594	NBP2-89696AFP594
Alexa Fluor Plus 647	NBP2-89696AFP647
Alexa Fluor Plus 680	NBP2-89696AFP680
Allophycocyanin	NBP2-89696APC
Biotin	NBP2-89696B
CoraFluor 1	NBP2-89696CL1
DyLight 350	NBP2-89696UV
DyLight 405	NBP2-89696V
DyLight 488	NBP2-89696G
DyLight 550	NBP2-89696R
DyLight 594	NBP2-89696DL594
DyLight 650	NBP2-89696C
DyLight 680	NBP2-89696FR
DyLight 755	NBP2-89696IR
FITC	NBP2-89696F
HRP	NBP2-89696H
Janelia Fluor 525	NBP2-89696JF525
Janelia Fluor 549	NBP2-89696JF549
Janelia Fluor 646	NBP2-89696JF646
Janelia Fluor 669	NBP2-89696JF669
PE	NBP2-89696PE
PerCP	NBP2-89696PCP
mFluor Violet 450 SE	NBP2-89696MFV450
mFluor Violet 500 SE	NBP2-89696MFV500
mFluor Violet 610 SE	NBP2-89696MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89696-50ul

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NBP3-28596

CD200/OX2 Antibody - IgG2SA, (Research Grade samalizumab Biosimilar) - Low Endotoxin, Azide and BSA Free

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28596AF350
Alexa Fluor 405	NBP3-28596AF405
Alexa Fluor 488	NBP3-28596AF488
Alexa Fluor 532	NBP3-28596AF532
Alexa Fluor 594	NBP3-28596AF594
Alexa Fluor 647	NBP3-28596AF647
Alexa Fluor 700	NBP3-28596AF700
Alexa Fluor 750	NBP3-28596AF750
Alexa Fluor Plus 405	NBP3-28596AFP405
Alexa Fluor Plus 488	NBP3-28596AFP488
Alexa Fluor Plus 555	NBP3-28596AFP555
Alexa Fluor Plus 594	NBP3-28596AFP594
Alexa Fluor Plus 647	NBP3-28596AFP647
Alexa Fluor Plus 680	NBP3-28596AFP680
Allophycocyanin	NBP3-28596APC
Biotin	NBP3-28596B
CoraFluor 1	NBP3-28596CL1
DyLight 350	NBP3-28596UV
DyLight 405	NBP3-28596V
DyLight 488	NBP3-28596G
DyLight 550	NBP3-28596R
DyLight 594	NBP3-28596DL594
DyLight 650	NBP3-28596C
DyLight 680	NBP3-28596FR
DyLight 755	NBP3-28596IR
FITC	NBP3-28596F
HRP	NBP3-28596H
Janelia Fluor 525	NBP3-28596JF525
Janelia Fluor 549	NBP3-28596JF549
Janelia Fluor 646	NBP3-28596JF646
Janelia Fluor 669	NBP3-28596JF669
PE	NBP3-28596PE
PerCP	NBP3-28596PCP
mFluor Violet 450 SE	NBP3-28596MFV450
mFluor Violet 500 SE	NBP3-28596MFV500
mFluor Violet 610 SE	NBP3-28596MFV610

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NBP2-90396

CD200/OX2 Antibody (002) - Azide and BSA Free

100 ul

NBP2-90396

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-90396AF350
Alexa Fluor 405	NBP2-90396AF405
Alexa Fluor 488	NBP2-90396AF488
Alexa Fluor 532	NBP2-90396AF532
Alexa Fluor 594	NBP2-90396AF594
Alexa Fluor 647	NBP2-90396AF647
Alexa Fluor 700	NBP2-90396AF700
Alexa Fluor 750	NBP2-90396AF750
Alexa Fluor Plus 405	NBP2-90396AFP405
Alexa Fluor Plus 488	NBP2-90396AFP488
Alexa Fluor Plus 555	NBP2-90396AFP555
Alexa Fluor Plus 594	NBP2-90396AFP594
Alexa Fluor Plus 647	NBP2-90396AFP647
Alexa Fluor Plus 680	NBP2-90396AFP680
Allophycocyanin	NBP2-90396APC
Biotin	NBP2-90396B
CoraFluor 1	NBP2-90396CL1
DyLight 350	NBP2-90396UV
DyLight 405	NBP2-90396V
DyLight 488	NBP2-90396G
DyLight 550	NBP2-90396R
DyLight 594	NBP2-90396DL594
DyLight 650	NBP2-90396C
DyLight 680	NBP2-90396FR
DyLight 755	NBP2-90396IR
FITC	NBP2-90396F
HRP	NBP2-90396H
Janelia Fluor 525	NBP2-90396JF525
Janelia Fluor 549	NBP2-90396JF549
Janelia Fluor 646	NBP2-90396JF646
Janelia Fluor 669	NBP2-90396JF669
PE	NBP2-90396PE
PerCP	NBP2-90396PCP
mFluor Violet 450 SE	NBP2-90396MFV450
mFluor Violet 500 SE	NBP2-90396MFV500
mFluor Violet 610 SE	NBP2-90396MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-90396-50ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

CD200/OX2 Antibodies

CD200/OX2 Antibody [Unconjugated]

CD200/OX2 Antibody [Unconjugated]

CD200/OX2 Antibody (325531) [Unconjugated]

CD200/OX2 Antibody (PSH01-48)

CD200/OX2 Antibody (325516) [Unconjugated]

CD200/OX2 Antibody (PSH01-49)

CD200/OX2 Antibody (OX-104) - BSA Free

CD200/OX2 Antibody (OX-2) - BSA Free

CD200/OX2 Antibody (6E8B11) - BSA Free

CD200/OX2 Antibody (333) - Azide and BSA Free

CD200/OX2 Antibody - IgG2SA, (Research Grade samalizumab Biosimilar) - Low Endotoxin, Azide and BSA Free

CD200/OX2 Antibody (002) - Azide and BSA Free