CD1b Antibody (CC20) - BSA Free

Immunohistochemistry: CD1b Antibody (CC20) [NB100-65315] - Detection of Mouse anti Bovine CD1w2 in an analysis on acetone-fixed frozen bovine thymus using the Histar detection system, original magnification x60.

Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and Mouse IgG2a isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody. Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and Mouse anti Bovine CD1w2 Antibody conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody.

Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse anti Bovine CD1w2 Antibody. All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes.

Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody and Mouse IgG2a isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody. Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody and Mouse anti Bovine CD1w2 Antibody conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody.

Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody and RPE conjugated Mouse anti Bovine CD1w2 Antibody.

• Reactivity: Bv, Ca, Eq, Gt, Sh
• Applications: Flow, IHC, IHC-Fr, IP
• Clone: CC20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD1b Antibody (CC20) - BSA Free Summary

• Immunogen: Made to Bovine CD1w2
• Specificity: Recognizes the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of 12kD and 46kD. CD1w2 is expressed by cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD1B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts with Bovine. Cross reacts with Sheep, Goat, Dog and Horse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD1b Antibody (CC20) - BSA Free

• CD1
• CD1A
• CD1b antigen
• CD1B antigen, b polypeptide
• CD1b molecule
• CD1b
• cortical thymocyte antigen CD1B
• differentiation antigen CD1-alpha-3
• MGC125990
• MGC125991
• R1
• T-cell surface glycoprotein CD1b

Background

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD1B