CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD1a Antibody (CBT6) - Chimeric [NBP3-12071] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of CBT6 (NBP3-12071) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12071, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free [NBP3-12071] - Molt4 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12071 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC, IP
• Clone: CBT6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free (NBP3-12071) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunizing mice with the human CD1a protein.
• Specificity: The antibody is specific for the human CD1a protein.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free

• CD1
• CD1a antigen
• CD1A antigen, a polypeptide
• CD1a molecule
• CD1a
• cluster of differentiation 1 A
• cortical thymocyte antigen CD1A
• differentiation antigen CD1-alpha-3
• epidermal dendritic cell marker CD1a
• FCB6
• hTa1 thymocyte antigen
• HTA1
• R4
• T6
• T-cell surface antigen T6/Leu-6
• T-cell surface glycoprotein CD1a

Background

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD1A