CD163 Antibody (R20) [Unconjugated]

Human peripheral blood monocytes were stained with Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and (A) Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB16072) or (B) isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: R20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD163 Antibody (R20) [Unconjugated] Summary

• Immunogen: Balb/c mice immunized with Synovial cells from Rheumatoid Arthritis patients
• Specificity: Detects human CD163 in Flow Cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD163 Antibody (R20) [Unconjugated]

• CD_antigen: CD163
• CD163 molecule
• CD163
• GHI/61
• HbSR
• Hemoglobin scavenger receptor
• M130
• macrophage-associated antigen
• MM130
• RM3/1
• SCARI1
• scavenger receptor cysteine-rich type 1 protein M130
• sCD163
• Soluble CD163

Background

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
4. Kristiansen, M. et al. (2001) Nature 409:198.
5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
6. Moller, H.J. et al. (2002) Blood 99:378.
7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
13. Schaer, D.J. et al. (2006) Blood 107:373.
14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
15. Fabriek, B.O. et al. (2007) Blood 109:5223.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.