Human CD163 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CD163 Quantikine ELISA Kit Summary

• Background: The Quantikine Human CD163 immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD163 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CD163 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD163 showed linear curves that were parallel to the standard curves obtained usi...ng the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD163.
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• Specificity: Natural and recombinant human CD163
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CD163

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD163 Quantikine ELISA Kit

• CD_antigen: CD163
• CD163 molecule
• CD163
• GHI/61
• HbSR
• Hemoglobin scavenger receptor
• M130
• macrophage-associated antigen
• MM130
• RM3/1
• SCARI1
• scavenger receptor cysteine-rich type 1 protein M130
• sCD163
• Soluble CD163

Background

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CD163 (DC1630)(49)

Publications using DC1630

• Jabbar, KS;Priya, S;Xu, J;Das Adhikari, U;Pishchany, G;Mohamed, ATM;Johansen, J;Thurimella, K;McCabe, C;Vlamakis, H;Okello, S;Delorey, TM;Lankowski, A;Mosepele, M;Siedner, MJ;Plichta, DR;Kwon, DS;Xavier, RJ; Human immunodeficiency virus and antiretroviral therapies exert distinct influences across diverse gut microbiomes Nature microbiology 2025-11-01 [PMID: 41168431] (Human)
• Martin, OP;Wallace, MS;Oetheimer, C;Patel, HB;Butler, MD;Wong, LP;Huang, P;Elbaz, J;Costentin, C;Salloum, S;Reinus, Z;Obinelo, A;Kim, U;Shroff, S;Corey, KE;Popov, YV;Charles, ED;Sadreyev, RI;Chung, RT;Alatrakchi, N; Single-cell atlas of human liver and blood immune cells across fatty liver disease stages reveals distinct signatures linked to liver dysfunction and fibrogenesis Nature immunology 2025-09-01 [PMID: 40883571] (Human)
• Pinto-Cardoso, S;Chávez-Torres, M;López-Filloy, M;Ávila-Ríos, S;Romero-Mora, K;Peralta-Prado, A; Patterns of immune recovery in people living with HIV who initiated antiretroviral therapy as late presenters BMC infectious diseases 2025-07-15 [PMID: 40665254] (Human)
• Reimer-McAtee, M;Serpa, J;McAtee, C;Ortega, E;Somasunderam, A;Arduino, R;Mejia, R;Utay, NS; Impact of intestinal parasitic infections on gut epithelial barrier and inflammation among foreign-born persons living with HIV medRxiv : the preprint server for health sciences 2025-02-05 [PMID: 39974003] (Human)
• Sturgeon, JP;Mutasa, K;Bwakura-Dangarembizi, M;Amadi, B;Ngosa, D;Dzikiti, A;Chandwe, K;Besa, E;Mutasa, B;Murch, SH;Hill, S;Playford, RJ;VanBuskirk, K;Kelly, P;Prendergast, AJ;TAME Trial Team, ; Therapeutic interventions targeting enteropathy in severe acute malnutrition modulate systemic and vascular inflammation and epithelial regeneration EBioMedicine 2024-12-10 [PMID: 39662176] (Human)
• Wang, P;Yang, L;Dong, J;Liu, W;Xie, F;Lu, Y;Li, W; The sEVs miR-487a/Notch2/GATA3 axis promotes osteosarcoma lung metastasis by inducing macrophage polarization toward the M2-subtype Cancer cell international 2024-08-31 [PMID: 39217351] (Human)
• Guan, X;Hu, R;Choi, Y;Srivats, S;Nabet, BY;Silva, J;McGinnis, L;Hendricks, R;Nutsch, K;Banta, KL;Duong, E;Dunkle, A;Chang, PS;Han, CJ;Mittman, S;Molden, N;Daggumati, P;Connolly, W;Johnson, M;Abreu, DR;Cho, BC;Italiano, A;Gil-Bazo, I;Felip, E;Mellman, I;Mariathasan, S;Shames, DS;Meng, R;Chiang, EY;Johnston, RJ;Patil, NS; Anti-TIGIT antibody improves PD-L1 blockade through myeloid and Treg cells Nature 2024-02-28 [PMID: 38418879] (Human)
• Rocafort, M;Gootenberg, DB;Luévano, JM;Paer, JM;Hayward, MR;Bramante, JT;Ghebremichael, MS;Xu, J;Rogers, ZH;Munoz, AR;Okello, S;Kim, JH;Sentongo, R;Wagubi, R;Lankowski, A;Maruapula, S;Zhao, G;Handley, SA;Mosepele, M;Siedner, MJ;Kwon, DS; HIV-associated gut microbial alterations are dependent on host and geographic context Nature communications 2024-02-05 [PMID: 38316748] (Human)
• Lischka, J;Schanzer, A;de Gier, C;Greber-Platzer, S;Zeyda, M; Macrophage-associated markers of metaflammation are linked to metabolic dysfunction in pediatric obesity Cytokine 2023-09-18 [PMID: 37729736] (Human)
• Chow, FC;Mundada, NS;Abohashem, S;La Joie, R;Iaccarino, L;Arechiga, VM;Swaminathan, S;Rabinovici, GD;Epel, ES;Tawakol, A;Hsue, PY; Psychological stress is associated with arterial inflammation in people living with treated HIV infection Brain, behavior, and immunity 2023-06-25 [PMID: 37369339] (Human)
• Kawanaka, M;Nishino, K;Kawada, M;Ishii, K;Tanikawa, T;Katsumata, R;Urata, N;Nakamura, J;Suehiro, M;Haruma, K;Kawamoto, H; Soluble CD163 is a predictor of fibrosis and hepatocellular carcinoma development in nonalcoholic steatohepatitis BMC gastroenterology 2023-05-10 [PMID: 37165352] (Human)
• Y Zhu, MJ Webster, AK Walker, P Massa, FA Middleton, CS Weickert Increased prefrontal cortical cells positive for macrophage/microglial marker CD163 along blood vessels characterizes a neuropathology of neuroinflammatory schizophrenia Brain, Behavior, and Immunity, 2023-03-25;0(0):. 2023-03-25 [PMID: 36972743] (Human)
• Y Zhu, MJ Webster, AK Walker, P Massa, FA Middleton, CS Weickert Increased prefrontal cortical cells positive for macrophage/microglial marker CD163 along blood vessels characterizes a neuropathology of neuroinflammatory schizophrenia Brain, Behavior, and Immunity, 2023;0(0):. 2023 [PMID: 36972743] (Human)
• LP Kincer, SB Joseph, MM Gilleece, BM Hauser, S Sizemore, S Zhou, C Di Germani, H Zetterberg, D Fuchs, SG Deeks, S Spudich, M Gisslen, RW Price, R Swanstrom Rebound HIV-1 in cerebrospinal fluid after antiviral therapy interruption is mainly clonally amplified R5 T cell-tropic virus Nature Microbiology, 2023-01-30;8(2):260-271. 2023-01-30 [PMID: 36717718] (Human)
• S Lee, N van den Be, A Castley, M Divitini, M Knuiman, P Price, D Nolan, F Sanfilippo, G Dwivedi Long-Term Associations between Human Cytomegalovirus Antibody Levels with All-Cause Mortality and Cardiovascular Outcomes in an Australian Community-Based Cohort Viruses, 2022-11-29;14(12):. 2022-11-29 [PMID: 36560680] (Human)
• RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis Nature Microbiology, 2022-12-15;0(0):. 2022-12-15 [PMID: 36522461] (Human)
• J Parantaine, G Barreto, R Koivuniemi, H Kautiainen, D Nordström, E Moilanen, M Hämäläinen, M Leirisalo-, K Nurmi, KK Eklund The biological activity of serum bacterial lipopolysaccharides associates with disease activity and likelihood of achieving remission in patients with rheumatoid arthritis Arthritis Research & Therapy, 2022-11-21;24(1):256. 2022-11-21 [PMID: 36411473] (Human)
• P Zuccalà, T Latronico, R Marocco, S Savinelli, S Vita, F Mengoni, T Tieghi, C Borgo, B Kertusha, A Carraro, G D'Ettorre, V Vullo, CM Mastroiann, GM Liuzzi, M Lichtner Longitudinal Assessment of Multiple Immunological and Inflammatory Parameters during Successful DAA Therapy in HCV Monoinfected and HIV/HCV Coinfected Subjects International Journal of Molecular Sciences, 2022-10-08;23(19):. 2022-10-08 [PMID: 36233234] (Human)
• Y Hara, J Tsukiji, A Yabe, Y Onishi, H Hirose, M Yamamoto, M Kudo, T Kaneko, T Ebina Heme oxygenase-1 as an important predictor of the severity of COVID-19 PLoS ONE, 2022-08-24;17(8):e0273500. 2022-08-24 [PMID: 36001619] (Human)
• M Vassallo, J Durant, R Fabre, L Lotte, A Sindt, A Puchois, A De Monte, R Cezar, P Corbeau, C Pradier Inflammatory Markers after Switching to a Dual Drug Regimen in HIV-Infected Subjects: A Two-Year Follow-Up Viruses, 2022-04-28;14(5):. 2022-04-28 [PMID: 35632669] (Human)
• JS Pedersen, MO Rygg, K Chrøis, EG Sustarsic, Z Gerhart-Hi, NJ Wever Albr, RR Serizawa, VB Kristianse, AL Basse, AEB Boilesen, BH Olsen, T Hansen, LL Gluud, S Madsbad, S Larsen, F Bendtsen, F Dela Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration Nature Communications, 2022-05-25;13(1):2931. 2022-05-25 [PMID: 35614135] (Human)
• T Gilada, SR Schnittman, E White, J Mercader, Y Wang, S Dasgupta, R Valdez, D Pinto-Sant, S Pasalar, J Sanchez, P Gonzales, JR Lama, R Bender Ign, A Duerr Immune Activation in Primary Human Immunodeficiency Virus: Influence of Duration of Infection, Treatment, and Substance Use Open forum infectious diseases, 2022-03-24;9(6):ofac155. 2022-03-24 [PMID: 35611350] (Human)
• LC Alexander, G McHorse, JL Huebner, AC Bay-Jensen, MA Karsdal, VB Kraus A matrix metalloproteinase-generated neoepitope of CRP can identify knee and multi-joint inflammation in osteoarthritis Arthritis Research & Therapy, 2021-08-31;23(1):226. 2021-08-31 [PMID: 34465395] (Human)
• MC Mueller, WV Kern, S Usadel, MC Pauly, T Cathomen, U Salzer Assessing the differential impact of chronic CMV and treated HIV infection on CD8+ T-cell differentiation in a matched cohort study: is CMV the key? AIDS research and therapy, 2021-06-30;18(1):37. 2021-06-30 [PMID: 34193181] (Human)
• R Cezar, A Winter, D Desigaud, M Pastore, L Kundura, AM Dupuy, C Cognot, T Vincent, C Reynes, C Dunyach-Re, JP Lavigne, R Sabatier, P Le Merre, E Maggia, P Corbeau Identification of distinct immune activation profiles in adult humans Scientific Reports, 2020-11-30;10(1):20824. 2020-11-30 [PMID: 33257766] (Human)
• JP Magalhães, DA Santos, IR Correia, M Hetheringt, R Ribeiro, JF Raposo, A Matos, MD Bicho, LB Sardinha Impact of combined training with different exercise intensities on inflammatory and lipid markers in type 2 diabetes: a secondary analysis from a 1-year randomized controlled trial Cardiovasc Diabetol, 2020-10-07;19(1):169. 2020-10-07 [PMID: 33028418] (Human)
• LB Giron, F Colomb, E Papasavvas, L Azzoni, X Yin, M Fair, A Anzurez, M Damra, K Mounzer, JR Kostman, P Tebas, U O'Doherty, H Tateno, Q Liu, MR Betts, LJ Montaner, M Abdel-Mohs Interferon-alpha alters host glycosylation machinery during treated HIV infection EBioMedicine, 2020-08-19;59(0):102945. 2020-08-19 [PMID: 32827942] (Human)
• LB Giron, CE Tanes, MH Schleimann, PA Engen, LM Mattei, A Anzurez, M Damra, H Zhang, K Bittinger, F Bushman, A Kossenkov, PW Denton, H Tateno, A Keshavarzi, AL Landay, M Abdel-Mohs Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection Mucosal Immunol, 2020-03-09;0(0):. 2020-03-09 [PMID: 32152415] (Human)
• JV Baker, J Wolfson, T Peterson, M Mooberry, M Gissel, H Mystakelis, MW Henderson, K Garcia-Mye, FS Rhame, TW Schacker, KE Brummel-Zi, I Sereti, NS Key, RP Tracy Factor Xa Inhibition Reduces Coagulation Activity but Not Inflammation Among People With HIV: A Randomized Clinical Trial Open Forum Infect Dis, 2020-02-01;7(2):ofaa026. 2020-02-01 [PMID: 32055640] (Human)
• Q Liu, Q Ou, H Chen, Y Gao, Y Liu, Y Xu, Q Ruan, W Zhang, L Shao Differential expression and predictive value of monocyte scavenger receptor CD163 in populations with different tuberculosis infection statuses BMC Infect. Dis., 2019-11-28;19(1):1006. 2019-11-28 [PMID: 31779590] (Human)
• G Guo, Y Zhu, Z Wu, H Ji, X Lu, Y Zhou, Y Li, X Cao, Y Lu, P Talbot, J Liao, Y Shi, H Bu Circulating monocytes accelerate acute liver failure by IL-6 secretion in monkey J. Cell. Mol. Med., 2018-07-11;0(0):. 2018-07-11 [PMID: 29992739] (Primate)
• C Mascia, I Pozzetto, B Kertusha, R Marocco, C Del Borgo, T Tieghi, S Vita, S Savinelli, M Iannetta, V Vullo, M Lichtner, CM Mastroiann Persistent high plasma levels of sCD163 and sCD14 in adult patients with measles virus infection PLoS ONE, 2018-05-24;13(5):e0198174. 2018-05-24 [PMID: 29795672] (Human)
• T Fujimura, Y Sato, K Tanita, Y Kambayashi, A Otsuka, Y Fujisawa, K Yoshino, S Matsushita, T Funakoshi, H Hata, Y Yamamoto, H Uchi, Y Nonomura, R Tanaka, M Aoki, K Imafuku, H Okuhira, S Furudate, T Hidaka, S Aiba Serum levels of soluble CD163 and CXCL5 may be predictive markers for immune-related adverse events in patients with advanced melanoma treated with nivolumab: a pilot study Oncotarget, 2018-02-15;9(21):15542-15551. 2018-02-15 [PMID: 29643991] (Human)
• M Keshtkar-J, M Ouyang, M Keshtkarja, S Almed, H Li, JD Walston, R Rios, SX Leng Effect of influenza vaccine on tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in older adults Vaccine, 2018-03-13;0(0):. 2018-03-13 [PMID: 29548604] (Human)
• LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa Reevaluation of immune activation in the era of cART and an aging HIV-infected population JCI Insight, 2017-10-19;2(20):. 2017-10-19 [PMID: 29046481] (Human)
• SG Al-Kindi, DA Zidar, GA McComsey, CT Longenecke Association of Anisocytosis with Markers of Immune Activation and Exhaustion in Treated HIV Pathog Immun, 2017-05-02;2(1):138-150. 2017-05-02 [PMID: 28713869] (Human)
• C Mascia, S Vita, P Zuccalà, R Marocco, T Tieghi, S Savinelli, R Rossi, M Iannetta, I Pozzetto, C Furlan, F Mengoni, CM Mastroiann, V Vullo, M Lichtner Changes in inflammatory biomarkers in HCV-infected patients undergoing direct acting antiviral-containing regimens with or without interferon PLoS ONE, 2017-06-21;12(6):e0179400. 2017-06-21 [PMID: 28636655] (Human)
• PA Alvarado-V, L Bernal, CA Paige, RL Grosick, C Moracho Vi, DW Ferreira, C Ulecia-Mor, EA Romero-San Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions Immunobiology, 2017-05-16;0(0):. 2017-05-16 [PMID: 28545809] (Human)
• M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection Open Forum Infect Dis, 2016-10-15;3(4):ofw224. 2016-10-15 [PMID: 28066794] (Human)
• Immune Activation at Sites of HIV/TB Co-Infection Contributes to the Pathogenesis of HIV-1 Disease PLoS ONE, 2016-11-21;11(11):e0166954. 2016-11-21 [PMID: 27870882] (Human)
• Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities. PLoS ONE, 2015-12-04;10(12):e0144317. 2015-12-04 [PMID: 26636578] (Human)
• Castley , Alison, Berry , Cassandr, French , Martyn, Fernandez , Sonia, Krueger , Romano, Nolan , David Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring? PLoS ONE, 2014-12-29;9(12):e115226. 2014-12-29 [PMID: 25544986] (Human)
• Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients. PLoS ONE, 2014-08-18;9(8):e105442. 2014-08-18 [PMID: 25133669] (Human)
• Beltran L, Munoz Hernandez R, de Pablo Bernal R, Garcia Morillo J, Egido J, Noval M, Ferrando-Martinez S, Blanco-Colio L, Genebat M, Villar J, Moreno-Luna R, Moreno J Reduced sTWEAK and increased sCD163 levels in HIV-infected patients: modulation by antiretroviral treatment, HIV replication and HCV co-infection. PLoS ONE, 2014-03-04;9(3):e90541. 2014-03-04 [PMID: 24594990] (Human)
• Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity. Arthritis Res Ther, 2013-01-01;15(6):R212. 2013-01-01 [PMID: 24325951] (Human)
• Forsvoll J, Kristoffersen E, Oymar K Elevated levels of CXCL10 in the Periodic Fever, Aphthous stomatitis, Pharyngitis and cervical Adenitis syndrome (PFAPA) during and between febrile episodes; an indication of a persistent activation of the innate immune system. Pediatr Rheumatol Online J, 2013-10-17;11(1):38. 2013-10-17 [PMID: 24134207] (Human)
• Subramanian K, Du R, Tan N, Ho B, Ding J CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms. J Immunol, 2013-04-15;190(10):5267-78. 2013-04-15 [PMID: 23589619] (Human)
• Shimizu M, Yachie A Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages. Cytokine, 2012-06-08;60(1):226-32. 2012-06-08 [PMID: 22682876] (Human)
• Moreno JA, Dejouvencel T, Labreuche J, Smadja DM, Dussiot M, Martin-Ventura JL, Egido J, Gaussem P, Emmerich J, Michel JB, Blanco-Colio LM, Meilhac O Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio. Arterioscler. Thromb. Vasc. Biol., 2010-03-18;30(6):1253-62. 2010-03-18 [PMID: 20299688] (Human)

Review for CD163 (DC1630) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 01/07/2021

Summary

• Sample Tested: Citrate Plasma,Human serum,patient CSF
• Lot: P251331

FAQs for CD163 (DC1630). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD163