CD163 Antibody (GHI/61) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Flow
Clone
GHI/61
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

CD163 Antibody (GHI/61) [Janelia Fluor® 646] Summary

Immunogen
The immunogen for this antibody was CD163.
Marker
Histiocytic Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD163
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
125.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD163 Antibody (GHI/61) [Janelia Fluor® 646]

  • CD_antigen: CD163
  • CD163 molecule
  • CD163
  • GHI/61
  • HbSR
  • Hemoglobin scavenger receptor
  • M130
  • macrophage-associated antigen
  • MM130
  • RM3/1
  • SCARI1
  • scavenger receptor cysteine-rich type 1 protein M130
  • sCD163
  • Soluble CD163

Background

CD163 (Cluster of Differentiation 163), also known by several other names including M130, p155, RM3/1, Ki-M8, Ber-MAC3, SM4, and GHI/61, is a type I transmembrane glycoprotein that is a member of the scavenger receptor cysteine-rich (SRCR) super family class B (1-3). CD163 is expressed specifically on the monocyte/macrophage lineage and has a theoretical molecular weight of 130-160 kDa in reducing conditions and 110 kDa in non-reducing conditions (2 - 6). CD163 is synthesized as 1076 amino acid (aa) protein consisting of a large extracellular domain (ECD) with nine SRCR domains, proline-serine-threonine rich (PST) linker domain, a transmembrane segment, and a cytoplasmic tail which has varying lengths depending on the isoform due to alternative splicing, with the short 49 aa form being the most common (1-3, 6).

One of the primary functions of CD163 is uptake of haptoglobin-hemoglobin (Hp-Hb) complexes from the liver, spleen, and bone marrow, ultimately triggering an anti-inflammatory response (3, 5, 7). CD163 also functions as an erythroblast adhesion receptor and promotes cell maturation and survival (3, 5, 7). Furthermore, CD163 functions in immune sensing of bacteria and as a receptor for tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) (3, 5, 7). As mentioned above, CD163 is expressed on cells in the monocyte/macrophage lineage and, in general, anti-inflammatory signals including glucocorticoids, interleukin (IL)-6, and IL-10 stimulate CD163 synthesis and expression while, conversely, pro-inflammatory signals such as interferon-gamma (INF-gamma), TNF-alpha, and lipopolysaccharide (LPS) downregulate CD163 (3, 5). In addition to membrane-bound form of CD163, the protein can be cleaved by metalloproteinases (MMP) and induced by LPS or phorbol myristate acetate (PMA) to release a soluble form (sCD163) into the plasma (7). Increased levels of sCD163 in the plasma and an increased number of CD163-expressing macrophages at the site of inflammation are associated with a variety of pathologies (3, 5-7). CD163/sCD163 is often increased and a suitable clinical marker for inflammatory diseases including rheumatoid arthritis (RA), Gaucher disease, chronic kidney disease, diabetes, and Crohn's disease (3, 5-7).

Alternative names for CD163 includes GHI/61, HbSR, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, RM3/1, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, sCD163, and soluble CD163.

References

1. Law, S. K., Micklem, K. J., Shaw, J. M., Zhang, X. P., Dong, Y., Willis, A. C., & Mason, D. Y. (1993). A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. European journal of immunology. https://doi.org/10.1002/eji.1830230940

2. Onofre, G., Kolackova, M., Jankovicova, K., & Krejsek, J. (2009). Scavenger receptor CD163 and its biological functions. Acta medica (Hradec Kralove).

3. Van Gorp, H., Delputte, P. L., & Nauwynck, H. J. (2010). Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy. Molecular immunology. https://doi.org/10.1016/j.molimm.2010.02.008

4. Sulahian, T. H., Hogger, P., Wahner, A. E., Wardwell, K., Goulding, N. J., Sorg, C., Droste, A., Stehling, M., Wallace, P. K., Morganelli, P. M., & Guyre, P. M. (2000). Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. https://doi.org/10.1006/cyto.2000.0720

5. Etzerodt, A., & Moestrup, S. K. (2013). CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxidants & redox signaling. https://doi.org/10.1089/ars.2012.4834

6. Skytthe, M. K., Graversen, J. H., & Moestrup, S. K. (2020). Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases. International journal of molecular sciences, 21(15), 5497. https://doi.org/10.3390/ijms21155497

7. Moller H. J. (2012). Soluble CD163. Scandinavian journal of clinical and laboratory investigation. https://doi.org/10.3109/00365513.2011.626868

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43341AF350
Alexa Fluor 405 NBP1-43341AF405
Alexa Fluor 488 NBP1-43341AF488
Alexa Fluor 532 NBP1-43341AF532
Alexa Fluor 594 NBP1-43341AF594
Alexa Fluor 647 NBP1-43341AF647
Alexa Fluor 700 NBP1-43341AF700
Alexa Fluor 750 NBP1-43341AF750
Allophycocyanin NBP1-43341APC
Allophycocyanin/Cy7 NBP1-43341APCCY7
Biotin NBP1-43341B
DyLight 350 NBP1-43341UV
DyLight 405 NBP1-43341V
DyLight 488 NBP1-43341G
DyLight 550 NBP1-43341R
DyLight 594 NBP1-43341DL594
DyLight 650 NBP1-43341C
DyLight 680 NBP1-43341FR
DyLight 755 NBP1-43341IR
FITC NBP1-43341F
HRP NBP1-43341H
Janelia Fluor 549 NBP1-43341JF549
Janelia Fluor 646 NBP1-43341JF646
PE NBP1-43341PE
PE/Atto594 NBP1-43341PEATT594
PE/Cy5.5 NBP1-43341PECY55
PE/Cy7 NBP1-43341PECY7
PerCP NBP1-43341PCP

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Bioinformatics

Gene Symbol CD163