CD161 Antibody (10/78)

Flow Cytometry: CD161 Antibody (10/78) [NB100-65297] - Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Monoclonal Antibody (Catalog# NB100-65297) or (B) Mouse IgG1 Isotype control (Catalog# MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog# F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Flow, IHC, IHC-Fr, IP
• Clone: 10/78
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CD161 Antibody (10/78) Summary

• Immunogen: Purified splenic NK cells from the LEW rat strain.
• Specificity: Recognizes the rat CD161 protein (also known as NKR-PI) a 60kD glycoprotein expressed on rat NK cells and T cell subpopulations. This competes with clone 3.2.3 for binding to antigen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLRB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD161 Antibody (10/78)

• CD161 antigen
• CD161
• CLEC5B
• CLEC5BC-type lectin domain family 5 member B
• HNKR-P1a
• killer cell lectin-like receptor subfamily B member 1
• killer cell lectin-like receptor subfamily B, member 1
• KLRB1
• Ly59
• Natural killer cell surface protein P1A
• NKR
• NKRP1
• NKR-P1
• NKRP1A
• NKR-P1AMGC138614
• NKRP1ANKR

Background

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD161 Antibody (NB100-65297) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Vincent Eckert IHC-P Rat 11/12/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Rat Lung Tissue,Rat liver
• Species: Rat
• Lot: CLCP02

Comments

• Comments: 1 star for using product in an unvalidated application IHC-P. Innovators Reward Program review.

FAQs for CD161 Antibody (NB100-65297). (Showing 1 - 1 of 1 FAQ).

1. I see that this antibody is listed for use in IHC...would that include IHC on formalin-fixed-paraffin-embedded tissue?
   • NB100-65297 has been tested and validated for use with frozen tissues however its performance has not been evaluated in paraffin embedded tissues yet and therefore cannot be guaranteed. If you would be interested in testing this antibody in IHC-Paraffin please take a look at our Innovators Reward Program.

Bioinformatics

• Gene Symbol: KLRB1
• Entrez:
  • 683758(Rat)
• Uniprot:
  • P27471(Rat)