CD161 Antibody (10/78) - Azide and BSA Free

Flow Cytometry: CD161 Antibody (10/78) - Azide and BSA Free [NBP2-80629] - Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Monoclonal Antibody (Catalog# NB100-65297) or (B) Mouse IgG1 Isotype control (Catalog# MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog# F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Flow, IHC, IP, CyTOF-ready
• Clone: 10/78
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD161 Antibody (10/78) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CD161 Antibody (10/78) - Azide and BSA Free (NB100-65297) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified splenic NK cells from the LEW rat strain.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLRB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD161 Antibody (10/78) - Azide and BSA Free

• CD161 antigen
• CD161
• CLEC5B
• CLEC5BC-type lectin domain family 5 member B
• HNKR-P1a
• killer cell lectin-like receptor subfamily B member 1
• killer cell lectin-like receptor subfamily B, member 1
• KLRB1
• Ly59
• Natural killer cell surface protein P1A
• NKR
• NKRP1
• NKR-P1
• NKRP1A
• NKR-P1AMGC138614
• NKRP1ANKR

Background

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLRB1