CD151 Antibody (455807) [Unconjugated]

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CD151 Monoclonal Antibody (Catalog # MAB4609, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).

CD151 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse CD151 Monoclonal Antibody (Catalog # MAB4609) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 455807
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD151 Antibody (455807) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with mouse CD151
  Met1-Tyr253
  Accession # BAA22447
• Specificity: Detects mouse CD151. Stains mouse CD151 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD151
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD151 Antibody (455807) [Unconjugated]

• CD151 antigen (Raph blood group)
• CD151 antigenplatelet surface glycoprotein gp27
• CD151 molecule (Raph blood group)
• CD151
• GP27
• Membrane glycoprotein SFA-1
• MER2
• PETA3
• PETA-3
• PETA-3SFA-1
• platelet-endothelial cell tetraspan antigen 3
• Platelet-endothelial tetraspan antigen 3
• RAPH
• SFA1
• SFA-1
• tetraspanin-24
• TSPAN24
• tspan-24
• TSPAN24hemidesmosomal tetraspanin CD151

Background

CD151 is a 29 kDa palmitoylated glycoprotein in the tetraspanin superfamily and is expressed by endothelial cells, epithelial cells, megakaryocytes, and platelets. It associates with other tetraspanin proteins and with some integrins including alpha 3/ beta 1, alpha 6/ beta 1, alpha 6/ beta 4, and alpha 7/ beta 1. CD151 modulates cellular adhesion, cell migration, and platelet activation. Mouse CD151 shares 94%-96% aa sequence identity with human and rat CD151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD151 Antibody (MAB4609)(1)

Publication using MAB4609

• Norman Sachs, Nike Claessen, Jan Aten, Maaike Kreft, Gwendoline J.D. Teske, Anneke Koeman et al. Blood pressure influences end-stage renal disease of Cd151 knockout mice Journal of Clinical Investigation 2012-01-03 [PMID: 22201679] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CD151
• Uniprot:
  • BAA22447()