CD11c Antibody (HC1/1)

Flow Cytometry: CD11c Antibody (HC1/1) [NBP2-32924] - Surface staining of human PBMCs using CD11c antibody at 1 ug/10^6 cells. this antibody and FACS surface staining kit this antibody were used for this test.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: HC1/1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD11c Antibody (HC1/1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34629)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human monocytic U937 phorbol ester-treated cells
• Localization: Cell surface
• Marker: Dendritic Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAX
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD11c Antibody (HC1/1)

• 95 alpha chain
• 95
• 95, alpha subunit
• CD11 antigen-like family member C
• CD11c antigen
• CD11c
• CD11Cleu M5, alpha subunit
• Integrin alpha X
• integrin alpha-X
• integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
• integrin, alpha X (complement component 3 receptor 4 subunit)
• ITGAX
• Leu M5
• Leukocyte adhesion glycoprotein p150
• Leukocyte adhesion receptor p150
• leukocyte surface antigen p150
• myeloid membrane antigen, alpha subunit
• p150 95 integrin alpha chain
• p150,95 alpha
• SLEB6

Background

CD11c encodes the integrin alpha X chain protein. CD11c is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAX
• Uniprot:
  • P20702(Human)