CD117/c-kit Antibody (YR145)

Product Discontinued

CD117/c-kit Antibody (YR145) Summary

• Immunogen: A synthetic peptide corresponding to residues in the C-terminus (cytoplasmic domain) of human c-Kit was used as an immunogen. The antibody does not cross-react with other CSF-1/PDGF receptor family member.
• Marker: Hematopoietic Stem Cell Marker
• Specificity: The antibody does not cross-react with other CSF-1/PDGF receptor family member.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KIT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-100
  • Immunohistochemistry-Paraffin 1:50-100
  • Western Blot 1:1000-10000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin. In Western blot this antibody detects a band at approximately 145kDa. This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)

Reactivity Notes

Cross reactivity determined by Western Blot only.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Culture supernatant in buffered aqueous solution
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for CD117/c-kit Antibody (YR145)

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Member of the Tyrosine Kinase Receptor (TKRs) and highly homologous to receptor PDF and CSF-1 (1-2), c-Kit is a cellular homologue of the oncogene v-Kit of the Hardy Zuckerman 4-feline sarcoma virus (3). Activation of c-Kit tyrosine kinase by SCF (Stem Cell factor) leads to autophosphorylation and association of c-Kit with substrate PI3K (4). Missense substitution within the cellular tyrosine kinase may cause Piebaldism an autosomal dominant disorder of the skin (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (NB110-55636)(4)

Publications using NB110-55636

• Sakamoto K, Sakurai S, Kanda T et al. Pleomorphic phenotypes of gastrointestinal stromal tumors at metastatic sites with or without imatinib treatment. Cancer Sci 2010-05-01 [PMID: 20180814] (IF/IHC)
• Davies MA, Stemke-Hale K, Lin E et al. Integrated Molecular and Clinical Analysis of AKT Activation in Metastatic Melanoma. Clin Cancer Res 2009-12-01 [PMID: 19996208] (WB, ICC/IF)
• Wong NA, Melegh Z et al. Antigen retrieval and primary antibody type affect sensitivity but not specificity of CD117 immunohistochemistry. Histopathology 2009-04-01 [PMID: 19413635] (IF/IHC)
• Saito M, Sakurai S, Motegi A et al. Comparative study using rabbit-derived polyclonal, mouse-derived monoclonal, and rabbit-derived monoclonal antibodies for KIT immunostaining in GIST and other tumors. Pathol Int 2007-04-01 [PMID: 17316415] (IF/IHC)

Bioinformatics

• Gene Symbol: KIT
• Entrez:
  • 3815(Human)
• Uniprot:
  • P10721()