CCR7 Antibody (Y59)

Product Discontinued

CCR7 Antibody (Y59) Summary

• Immunogen: A synthetic peptide corresponding to residues in N-terminal extracellular domain of human CKR7 was used as immunogen.
• Specificity: The antibody does not cross-react with other G-protein coupled receptor 1 family members.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCR7
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 1:10
  • Western Blot 1:1000-10000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. Immunohistochemistry-Frozen was reported in scientific literature. In Western blot this antibody detects a band at approximately 45kDa.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

May cross reacts with Rhesus monkey.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for CCR7 Antibody (Y59)

• BLR2
• BLR2C-C chemokine receptor type 7
• C-C CKR-7
• CC-CKR-7
• CCR7
• CCR-7
• CD197 antigen
• CD197
• CDw197
• CDw197CC chemokine receptor 7
• chemokine (C-C motif) receptor 7
• CMKBR7
• CMKBR7chemokine (C-C) receptor 7
• EBI1
• EBI1EBV-induced G protein-coupled receptor 1
• EBV-induced G-protein coupled receptor 1
• Epstein-Barr virus induced gene 1
• Epstein-Barr virus induced G-protein coupled receptor
• Epstein-Barr virus-induced G-protein coupled receptor 1
• EVI1
• lymphocyte-specific G protein-coupled peptide receptor
• MIP-3 beta receptor

Background

Chemokine receptor 7 (CCR7) is a G-protein coupled receptor (GPCR) that binds chemokine ligand 19 (CCL19) and chemokine ligand 21 (CCL21) and, together, this receptor-ligand interaction functions in inflammatory and adaptive immune response (1,2). The CCR7 protein contains 7-transmembrane spanning alpha helices and is 378 amino acids (aa) in length, with a theoretical molecular weight of 42.8 kDa (3,4). CCR7 is expressed on several cells within the immune system, including naive T cells, central memory T cells, regulatory T cells, naive B cells, a subset of double negative and single positive thymocytes, and mature dendritic cells (DCs) (1, 3).

The primary role of the CCR7/CCL19/CCL21 chemokine signaling axis is homing T cells and DCs to lymph nodes and lymphoid tissues to initiate an immune response (1,2,5,6). In the context of cancer, the CCR7 signaling axis appears to have two opposing roles (2). Downregulation of CCR7 on CD8+ T cells contributes to effector cell migration and anti-cancer activities via cytotoxic tumor-infiltrating lymphocytes (2). However, upregulation of CCR7 by cancer cells can result in cancer cell migration and metastasis (2). Overexpression of CCR7 has been implicated in a variety of cancers including breast, cervical, gastric, head and neck cell carcinoma, and prostate (1,2,7). Studies in breast cancer have found that hypoxia increases CCR7 expression, and this activation can affect cancer cell invasion, extravasation, proliferation, angiogenesis, and metastasis through induction of multiple signaling transduction pathways such as PI3K/AKT, MAPK, and JAK/STAT (5,7).

Given its important role in inflammation and immune response, several strategies have been employed to target the CCR7 signaling axis for cancer immunotherapy (2). Some cancer immunotherapies under investigation include intra-tumoral administration of CCL19 and CCL21, introduction of patient-derived cells transfected to express CCR7 or its ligands, and vaccines (2). Further interrogation of CCR7/CCL19/CCL21 signaling axis is required to develop better therapeutic strategies for cancer treatment.

References:

1. Comerford, I., Harata-Lee, Y., Bunting, M. D., Gregor, C., Kara, E. E., & McColl, S. R. (2013). A myriad of functions and complex regulation of the CCR7/CCL19/CCL21 chemokine axis in the adaptive immune system. Cytokine & growth factor reviews, 24(3), 269-283. https://doi.org/10.1016/j.cytogfr.2013.03.001

2. Salem, A., Alotaibi, M., Mroueh, R., Basheer, H. A., & Afarinkia, K. (2021). CCR7 as a therapeutic target in Cancer. Biochimica et biophysica acta. Reviews on cancer, 1875(1), 188499. https://doi.org/10.1016/j.bbcan.2020.188499

3. Yan, Y., Chen, R., Wang, X., Hu, K., Huang, L., Lu, M., & Hu, Q. (2019). CCL19 and CCR7 Expression, Signaling Pathways, and Adjuvant Functions in Viral Infection and Prevention. Frontiers in cell and developmental biology, 7, 212. https://doi.org/10.3389/fcell.2019.00212

4. Uniprot (P32248)

5. Korbecki, J., Grochans, S., Gutowska, I., Barczak, K., & Baranowska-Bosiacka, I. (2020). CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of Receptors CCR5, CCR6, CCR7, CCR8, CCR9, and CCR10 Ligands. International journal of molecular sciences, 21(20), 7619. https://doi.org/10.3390/ijms21207619

6. Sanchez-Sanchez, N., Riol-Blanco, L., & Rodriguez-Fernandez, J. L. (2006). The multiple personalities of the chemokine receptor CCR7 in dendritic cells. Journal of immunology (Baltimore, Md. : 1950), 176(9), 5153-5159. https://doi.org/10.4049/jimmunol.176.9.5153

7. Rizeq, B., & Malki, M. I. (2020). The Role of CCL21/CCR7 Chemokine Axis in Breast Cancer Progression. Cancers, 12(4), 1036. https://doi.org/10.3390/cancers12041036

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR7 Antibody (NB110-55680)(9)

Publications using NB110-55680

• Finetti F, Capitani N, Manganaro N et al. Optimization of Organotypic Cultures of Mouse Spleen for Staining and Functional Assays Front Immunol 2020-03-24 [PMID: 32265925] (FLOW, Mouse)
• Patrussi L, Capitani N, Ulivieri C et al. p66Shc deficiency in the Eu-TCL1 mouse model of chronic lymphocytic leukemia enhances leukemogenesis by altering the chemokine receptor landscape Haematologica 2019-02-28 [PMID: 30819907] (WB, Human)
• Finsen Alexandra Vanessa, Ueland Thor, Sjaastad Ivar et al. The homeostatic chemokine CCL21 predicts mortality in aortic stenosis patients and modulates left ventricular remodeling. PLoS One 2014-01-01 [PMID: 25398010] (ICC/IF, ICC/IF, Human)
• Sugg Kristoffer B, Lubardic Jovan, Gumucio Jonathan P, Mendias Christopher L. Changes in macrophage phenotype and induction of epithelial-to-mesenchymal transition genes following acute Achilles tenotomy and repair. J Orthop Res. 2014-04-04 [PMID: 24700411] (Rat)
• Astrup Elisabeth, Ranheim Trine, Damas Jan K et al. Increased expression of the homeostatic chemokines CCL19 and CCL21 in clinical and experimental Rickettsia conorii infection. BMC Infect Dis. 2014-02-09 [PMID: 24507453] (ICC/IF)
• Janairo RR, Zhu Y, Chen T, Li S. Mucin Covalently Bonded to Microfibers Improves the Patency of Vascular Grafts. Tissue Eng Part A. 2013-08-21 [PMID: 23962121] (IHC-Fr, ICC/IF, Rat)
• Lee BL, Jeon H, Wang A, Yan Z, Yu J, Grigoropoulos C, Li S. Femtosecond laser ablation enhances cell infiltration into three-dimensional electrospun scaffolds. Acta Biomater. 2012-04-19 [PMID: 22522128] (IHC-Fr, ICC/IF, Rat)
• Rebhun RB, Cheng H, Gershenwald JE et al. Constitutive expression of the alpha4 integrin correlates with tumorigenicity and lymph node metastasis of the B16 murine melanoma. Neoplasia 2010-02-01 [PMID: 20126475] (WB)
• Gottfried-Blackmore A, Kaunzner UW, Idoyaga J et al. Acute in vivo exposure to interferon-? enables resident brain dendritic cells to become effective antigen presenting cells . Proc Natl Acad Sci U S A 106 20918-10923. 2009-01-01 [PMID: 19906988] (WB)

Bioinformatics

• Gene Symbol: CCR7
• Entrez:
  • 1236(Human)
  • 12775(Mouse)
  • 287673(Rat)
• Uniprot:
  • P32248()