CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free

Western Blot: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Jurkat (A), Molt4 (B), K562 (C) and Daudi (D) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RC-10 (NBP3-12000) at 0.0003 ug/ml, 0.001 ug/ml, 0.003 ug/ml and unknown ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RC-10 (NBP3-12000) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12000, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free [NBP3-12000] - Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12000 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: RC-10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunizing mice with CCR5.
• Specificity: This antibody recognizes non-activated nonphospho-CCR5. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCR5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• chemr13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

C-C chemokine receptor type 5 (CCR5) is a member of the beta chemokine receptors family of integral membrane proteins. The CCR5 protein is a G protein-coupled receptor which functions as a chemokine receptor in the CC chemokine group.

CCR5 is a major coreceptor for human HIV infection and is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.

CCR5 antibodies are useful for HIV infection studies and research on immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCR5