CCR3 Antibody Blocking Peptide

Product Discontinued

CCR3 Antibody Blocking Peptide Summary

• Description: A CCR3 antibody blocking peptide.
• Immunogen: Synthetic peptides taken form near N-terminal region of the human CCR3 protein from within aa region 1-50. The peptide was post-synthetically modified and conjugated to carrier protein to achieve desired antigenicity.
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: CCR3

Applications/Dilutions

• Dilutions:
  • Antibody Competition
• Application Notes: This peptide is useful as a blocking peptide for NBP1-78223. The CCR3 peptide sequence was conserved in human, gibbon, chimp, and monkey but not in other species.
  
  For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.

Alternate Names for CCR3 Antibody Blocking Peptide

• b-chemokine receptor
• CC chemokine receptor 3
• C-C chemokine receptor type 3
• C-C CKR-3
• CCR3
• CCR-3
• CD193 antigen
• CD193
• chemokine (C-C motif) receptor 3
• CKR3CC-CKR-3CMKBR3CD193
• eosinophil CC chemokine receptor 3
• Eosinophil eotaxin receptor
• MGC102841

Background

Chemokine receptors represent a subfamily of ~20 GPCRs that were originally identified by their roles in immune cell trafficking. Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and RANTES, members of the beta chemokine family of leukocyte chemo-attractants, bind to a common seven-transmembrane-domain human receptor. Chemokine (Chemo-attractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CCR) belong to the superfamily of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and directing the proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases. Induction of chemokines and infiltration of chemokine receptor-bearing cells has also been shown in a variety of animal models of renal diseases, as well as in human diseases and allograft rejection (2) Esinophil eotaxin is the ligand for CCR3, upon activation CCR3 undergo degranulation, chemotaxis and exhibit Ca+2 changes in response to human CC chemokine macrophage inflammatory protein-1 alpha (MIP1alpha), RANTES and monocyte chemo-attractant protein MCP3 (MCP-3). CCR3 is most closely related and similar to CCR1. Activation of CCR3 lead to selective recruitment of eosinophils to the site of inflammation (3). CCR3 is a multi-pass membrane protein, containing 7TMDs with apparent MW of 46kDa (373 aa) expressed on many different blood cells. The CCR2 protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins. The short carboxy terminal is intracellular and has putative post-translational sites. Over expression of CCR3 along with its ligand appears to be chracterics of ulcerative colitis. The production of CCR3 ligands by human colonic epithelial cells suggests further that the epithelium can play a role in modulating pathological T cell-mediated mucosal inflammation. The Anti-CCR3-selective antibodies were generated against conserved but unique sequences from N-terminal region of human CCR3 receptor proteins. The peptide sequence resides between aa 1-50 aa of human CCR3 and are unique to CCR3 protein. Antibodies generated against this peptide are specific for CCR3, the CCR3 peptide antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. The affinity purified antibodies are also conjugated to FITC and biotin for direct applications in IHC and cell sorting experiments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCR3