CCR1 Antibody

Western blot shows lysates of mouse heart tissue and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse CCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5986) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse CCR1
  Met1-Ala34, Asp92-Lys107, Phe172-Arg197, Gln265-Leu281
  Accession # AAH11092
• Specificity: Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) CCR4, rmCCR5, and rmCCR8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CCR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR1 Antibody

• C-C chemokine receptor type 1
• CC-CKR-1
• CCR1
• CCR-1
• CD191 antigen
• CD191
• chemokine (C-C motif) receptor 1
• CKR1
• CKR-1
• CMKBR1SCYAR1
• CMKR1
• HM145C-C CKR-1
• LD78 receptor
• Macrophage inflammatory protein 1-alpha receptor
• MIP-1alpha-R
• MIP1aR
• RANTES receptor
• RANTES-R

Background

CCR1 (C-C chemokine receptor 1; also MIP-1 alpha receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13⁺ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1 alpha /CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent agonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a 50 aa C‑terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCR1
• Uniprot:
  • AAH11092()