CCR1 Antibody (643854) [Unconjugated]

Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCR1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: 643854
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR1 Antibody (643854) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCR1
  Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)
  Accession # P51675
• Specificity: Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR1 Antibody (643854) [Unconjugated]

• C-C chemokine receptor type 1
• CC-CKR-1
• CCR1
• CCR-1
• CD191 antigen
• CD191
• chemokine (C-C motif) receptor 1
• CKR1
• CKR-1
• CMKBR1SCYAR1
• CMKR1
• HM145C-C CKR-1
• LD78 receptor
• Macrophage inflammatory protein 1-alpha receptor
• MIP-1alpha-R
• MIP1aR
• RANTES receptor
• RANTES-R

Background

CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13⁺ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a
50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR1 Antibody (MAB5986)(4)

Publications using MAB5986

• Batudeligen, ;Han, Z;Chen, H;Narisu, ;Xu, Y;Anda, ;Han, G; Luteolin Alleviates Liver Fibrosis in Rat Hepatic Stellate Cell HSC-T6: A Proteomic Analysis Drug design, development and therapy 2023-06-17 [PMID: 37360572] (Western Blot, Rat)
• C Xie, F Ye, N Zhang, Y Huang, Y Pan, X Xie CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype Journal of Cellular and Molecular Medicine, 2021-06-29;0(0):. 2021-06-29 [PMID: 34189838] (Western Blot, Mouse)
• Lewis , Nuruddee, Muthukumarana , Akalushi, Fogal , Steven E, Corradini , Laura, Stefanopoulos , Dimitria, Adusumalli , Prathima, Pelletier , Josephin, Panzenbeck , Mark, Berg , Karen, Canfield , Melissa, Cook , Brian N, Razavi , Hossein, Kuzmich , Daniel, Anderson , Shawn, Allard , Devan, Harrison , Paul, Grimaldi , Christin, Souza , Donald, Harcken , Christia, Fryer , Ryan M, Modis , Louise K, Brown , Maryanne CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells. PLoS ONE, 2014-08-29;9(8):e105883. 2014-08-29 [PMID: 25170619] (Flow Cytometry, Mouse)
• Baba T, Naka K, Morishita S, Komatsu N, Hirao A, Mukaida N MIP-1alpha/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia. J Exp Med, 2013-10-28;210(12):2661-73. 2013-10-28 [PMID: 24166712] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CCR1
• Uniprot:
  • P51675()