CCR1 Antibody (643854) [Phycoerythrin]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5986P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

L1.2 mouse pro-B cell line transfected with mouse CCR1 and eGFP was stained with Rat Anti-Mouse CCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5986P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 643854
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

CCR1 Antibody (643854) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant mouse CCR1
  Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)
  Accession # P51675
• Specificity: Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR1 Antibody (643854) [Phycoerythrin]

• C-C chemokine receptor type 1
• CC-CKR-1
• CCR1
• CCR-1
• CD191 antigen
• CD191
• chemokine (C-C motif) receptor 1
• CKR1
• CKR-1
• CMKBR1SCYAR1
• CMKR1
• HM145C-C CKR-1
• LD78 receptor
• Macrophage inflammatory protein 1-alpha receptor
• MIP-1alpha-R
• MIP1aR
• RANTES receptor
• RANTES-R

Background

CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13⁺ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR1 Antibody (FAB5986P)(7)

Publications using FAB5986P

• Alvaro Morales-Molina, Miguel Ángel Rodriguez-Milla, Stefano Gambera, Teresa Cejalvo, Belén de Andrés, María-Luisa Gaspar et al. Toll-like Receptor Signaling–deficient Cells Enhance Antitumor Activity of Cell-based Immunotherapy by Increasing Tumor Homing Cancer Research Communications 2023-03-01 [PMID: 36875156] (Flow Cytometry, Mouse)
• Alvaro Morales-Molina, Miguel Ángel Rodriguez-Milla, Stefano Gambera, Teresa Cejalvo, Belén de Andrés, María-Luisa Gaspar et al. Toll-like Receptor Signaling–deficient Cells Enhance Antitumor Activity of Cell-based Immunotherapy by Increasing Tumor Homing Cancer Research Communications 3/1/2023 [PMID: 36875156]
• S Trittel, BJ Chambers, U Heise, CA Guzmán, P Riese Key features and homing properties of NK cells in the liver are shaped by activated iNKT cells Sci Rep, 2019-11-08;9(1):16362. 2019-11-08 [PMID: 31704965] (Flow Cytometry, Mouse)
• Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced anti-viral therapeutic effects Sci Rep, 2016-12-13;6(0):39071. 2016-12-13 [PMID: 27958383] (Flow Cytometry, Mouse)
• Fleming B, Chandrasekaran P, Dillon L, Dalby E, Suresh R, Sarkar A, El-Sayed N, Mosser D The generation of macrophages with anti-inflammatory activity in the absence of STAT6 signaling. J Leukoc Biol, 2015-06-05;98(3):395-407. 2015-06-05 [PMID: 26048978] (Flow Cytometry, Mouse)
• Michail S. Lionakis, Muthulekha Swamydas, Brett G. Fischer, Theo S. Plantinga, Melissa D. Johnson, Martin Jaeger et al. CX3CR1-dependent renal macrophage survival promotes Candida control and host survival Journal of Clinical Investigation 2013-12-02 [PMID: 24177428] (Flow Cytometry, Transgenic Mouse)
• Michail S. Lionakis, Brett G. Fischer, Jean K. Lim, Muthulekha Swamydas, Wuzhou Wan, Chyi-Chia Richard Lee et al. Chemokine Receptor Ccr1 Drives Neutrophil-Mediated Kidney Immunopathology and Mortality in Invasive Candidiasis PLoS Pathogens 2012-08-16 [PMID: 22916017]

Bioinformatics

• Gene Symbol: CCR1
• Uniprot:
  • P51675()