CCR1 Antibody (53504) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB145P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 53504
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CCR1 Antibody (53504) [Phycoerythrin] Summary

• Additional Information: Clone 53504 was used by HLDA to establish CD designation
• Immunogen: Swiss 3T3 mouse cell line transfected with human CCR1
  Accession # P32246
• Specificity: Detects human CCR1. Reacts specifically with human CCR1 transfectants and not the parental cell line in flow cytometry. No cross‑reactivity with CCR5 transfectants is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR1 Antibody (53504) [Phycoerythrin]

• C-C chemokine receptor type 1
• CC-CKR-1
• CCR1
• CCR-1
• CD191 antigen
• CD191
• chemokine (C-C motif) receptor 1
• CKR1
• CKR-1
• CMKBR1SCYAR1
• CMKR1
• HM145C-C CKR-1
• LD78 receptor
• Macrophage inflammatory protein 1-alpha receptor
• MIP-1alpha-R
• MIP1aR
• RANTES receptor
• RANTES-R

Background

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR1 Antibody (FAB145P)(26)

Publications using FAB145P

• Martin J. Scott, Amanda Jowett, Martin Orecchia, Peter Ertl, Larissa Ouro-Gnao, Julia Ticehurst et al. Rapid identification of highly potent human anti-GPCR antagonist monoclonal antibodies mAbs 2020-04-29 [PMID: 32343620]
• E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis Arthritis Res. Ther., 2018-06-07;20(1):114. 2018-06-07 [PMID: 29880013] (Flow Cytometry, Human)
• Lee Faulkner Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity Toxicol. Sci, 2016-09-16;154(2):416-429. 2016-09-16 [PMID: 27637899] (Flow Cytometry, Human)
• Geng D, Kaczanowska S, Tsai A, Younger K, Ochoa A, Rapoport A, Ostrand-Rosenberg S, Davila E TLR5 Ligand-Secreting T Cells Reshape the Tumor Microenvironment and Enhance Antitumor Activity. Cancer Res, 2015-03-20;75(10):1959-71. 2015-03-20 [PMID: 25795705] (Flow Cytometry, Human)
• Broux B, Mizee M, Vanheusden M, van der Pol S, van Horssen J, Van Wijmeersch B, Somers V, de Vries H, Stinissen P, Hellings N IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis. J Immunol, 2015-01-23;194(5):2099-109. 2015-01-23 [PMID: 25617471] (Flow Cytometry, Human)
• Morandi , Fabio, Di Carlo , Emma, Ferrone , Soldano, Petretto , Andrea, Pistoia , Vito, Airoldi , Irma IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol, 2014-02-19;192(6):2634-42. 2014-02-19 [PMID: 24554774] (Flow Cytometry, Human)
• Kenneth Ky Liu, Katerina Dorovini-Zis Differential Regulation of CD4+ T Cell Adhesion to Cerebral Microvascular Endothelium by the beta -Chemokines CCL2 and CCL3 International Journal of Molecular Sciences 2012-11-30 [PMID: 23203188]
• Vujanovic , Lazar, Ballard , Wenners, Thorne , Stephen, Vujanovic , Nikola L, Butterfield , Lisa H Adenovirus-engineered human dendritic cells induce natural killer cell chemotaxis via CXCL8/IL-8 and CXCL10/IP-10. Oncoimmunology, 2012-07-01;1(4):448-457. 2012-07-01 [PMID: 22754763] (Flow Cytometry, Human)
• Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011-09-01;6(9):e23741. 2011-09-01 [PMID: 21912642] (Flow Cytometry, Human)
• Julie Catusse, Marion Leick, Mareike Groch, David J Clark, Maike V Buchner, Katja Zirlik et al. Role of the atypical chemoattractant receptor CRAM in regulating CCL19 induced CCR7 responses in B-cell chronic lymphocytic leukemia Molecular Cancer 2010-12-01 [PMID: 21092185]
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Carbone T, Nasorri F, Pennino D CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses. J. Immunol., 2009-12-11;184(2):1102-10. 2009-12-11 [PMID: 20008290] (Flow Cytometry, Human)
• Barroso-Gonzalez J, El Jaber-Vazdekis N, Garcia-Exposito L, Machado JD, Zarate R, Ravelo AG, Estevez-Braun A, Valenzuela-Fernandez A The Lupane-type Triterpene 30-Oxo-calenduladiol Is a CCR5 Antagonist with Anti-HIV-1 and Anti-chemotactic Activities. J. Biol. Chem., 2009-04-22;284(24):16609-20. 2009-04-22 [PMID: 19386595] (Flow Cytometry, Human)
• Ahern D, Lloyd CM, Robinson DS Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors. Allergy, 2009-02-07;64(8):1121-9. 2009-02-07 [PMID: 19208087] (Flow Cytometry, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, Human)
• Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008-06-30;84(4):1109-19. 2008-06-30 [PMID: 18591415] (Flow Cytometry, Human)
• Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation. J. Neuroimmunol., 2008-06-09;199(1):35-45. 2008-06-09 [PMID: 18538864] (Flow Cytometry, Human)
• Pokkali S, Das SD, R L Expression of CXC and CC type of chemokines and its receptors in tuberculous and non-tuberculous effusions. Cytokine, 2008-01-28;41(3):307-14. 2008-01-28 [PMID: 18226915] (Flow Cytometry, Human)
• Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood, 2007-04-01;109(7):2936-43. 2007-04-01 [PMID: 17132726] (Flow Cytometry, Human)
• Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy. Int. J. Cancer, 2006-05-01;118(9):2147-57. 2006-05-01 [PMID: 16331601] (Flow Cytometry, Human)
• Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation. Blood, 2004-09-28;105(3):931-9. 2004-09-28 [PMID: 15454484] (Flow Cytometry, Human)
• Chang WL, Baumgarth N, Yu D, Barry PA Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. J. Virol., 2004-08-01;78(16):8720-31. 2004-08-01 [PMID: 15280480] (Flow Cytometry, Human)
• Casazza S, Zappia E, Pistorio A, Gambini C Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Proc. Natl. Acad. Sci. U.S.A., 2004-07-19;101(30):11064-9. 2004-07-19 [PMID: 15263096] (Flow Cytometry, Human)
• Liu LY, Jarjour NN, Busse WW, Kelly EA Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge. J. Allergy Clin. Immunol., 2003-09-01;112(3):556-62. 2003-09-01 [PMID: 13679815] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-05-01;73(5):584-90. 2003-05-01 [PMID: 12714572] (Flow Cytometry, Human)
• Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces. Eur J Immunol, 2002-01-01;32(1):231-42. 2002-01-01 [PMID: 11782014] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR1
• Uniprot:
  • P32246()