Guinea Pig CCL5/RANTES ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
0.156 ng/ml-10 ng/ml |
| Sensitivity |
0.081 ng/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10% |
| Intra-Assay |
CV% < 8% |
| Sample Volume |
50-100ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CCL5 |
Applications/Dilutions
Reactivity Notes
Please contact our Technical Services with any questions regarding species reactivity
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Adhesive Strip (For 96 wells)
- Coated assay plate
- HRP-conjugate
- Instruction manual
- Negative Control
- Positive Control
- Sample Diluent
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (30 x concentrate)
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Alternate Names for Guinea Pig CCL5/RANTES ELISA Kit (Colorimetric)
Background
This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for CCL5/RANTES ELISA Kit (NBP2-59970). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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