CCL5/RANTES Antibody (21445) [Unconjugated]

Recombinant Human CCL5/RANTES (Catalog #278-RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND50 is typically0.1-0.4 µg/mL.

CCL5/RANTES was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, B/N, CyTOF-ready, Flow
• Clone: 21445
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL5/RANTES Antibody (21445) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL5/RANTES
• Specificity: Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4, recombinant mouse (rm) CCL3, and rmCCL4.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL5
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.1-0.4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL5/RANTES Antibody (21445) [Unconjugated]

• CCL5
• chemokine (C-C motif) ligand 5
• D17S136Enormally T-expressed, and presumably secreted
• EoCP
• Eosinophil chemotactic cytokine
• RANTES
• SISd
• SIS-delta
• small inducible cytokine A5 (RANTES)
• small inducible cytokine subfamily A (Cys-Cys), member 5
• Small-inducible cytokine A5
• T cell-specific protein P228
• T-cell specific protein p288
• TCP228T-cell-specific protein RANTES

Background

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL5/RANTES Antibody (MAB278)(15)

Publications using MAB278

• Parween, F;Singh, SP;Kathuria, N;Zhang, HH;Ashida, S;Otaizo-Carrasquero, FA;Shamsaddini, A;Gardina, PJ;Ganesan, S;Kabat, J;Lorenzi, HA;Riley, DJ;Myers, TG;Pittaluga, S;Bielekova, B;Farber, JM; Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines Nature communications 2025-02-26 [PMID: 40000641] (Immunocytochemistry, Human)
• F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (ICC, Human)
• Gemma Sullivan, Paola Galdi, Nis Borbye-Lorenzen, David Q. Stoye, Gillian J. Lamb, Margaret J. Evans et al. Preterm Birth Is Associated With Immune Dysregulation Which Persists in Infants Exposed to Histologic Chorioamnionitis Frontiers in Immunology 8/27/2021 [PMID: 34512648]
• Choi YW, Kim YH, Oh SY et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer Advanced Science 1/4/2021 [PMID: 33643790]
• AA de Cubas, W Dunker, A Zaninovich, RA Hongo, A Bhatia, A Panda, KE Beckermann, G Bhanot, S Ganesan, J Karijolich, WK Rathmell DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer JCI Insight, 2020-06-04;5(11):. 2020-06-04 [PMID: 32493845] (Neutralization, Human)
• Lixia Xu, Zhenwei Li, Sirong Song, Qian Chen, Lidong Mo, Chen Wang et al. Downregulation of alpha ‐ l ‐fucosidase 1 suppresses glioma progression by enhancing autophagy and inhibiting macrophage infiltration Cancer Science 7/1/2020 [PMID: 32314457]
• SK Singh, MK Mishra, BM Rivers, JB Gordetsky, S Bae, R Singh Biological and Clinical Significance of the CCR5/CCL5 Axis in Hepatocellular Carcinoma Cancers (Basel), 2020-04-05;12(4):. 2020-04-05 [PMID: 32260550] (IHC, Human)
• Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam. Mod Pathol, 2012-11-23;26(3):357-69. 2012-11-23 [PMID: 23174938] (IHC-P, Human)
• Michelle L. Scalley-Kim, Bruce W. Hess, Ryan L. Kelly, Anne-Rachel F. Krostag, Kurt H. Lustig, John S. Marken et al. A Novel Highly Potent Therapeutic Antibody Neutralizes Multiple Human Chemokines and Mimics Viral Immune Modulation PLoS ONE 8/17/2012 [PMID: 22912856]
• van Bladel ER, Roest M Up-regulation of platelet activation in hemophilia A. Haematologica, 2011-03-21;0(0):. 2011-03-21 [PMID: 21422116] (ELISA Development, Human)
• Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua. J. Pathol., 2008-02-01;214(3):328-36. 2008-02-01 [PMID: 18069648] (ELISA Development, IHC-Fr, Human)
• Bolovan-Fritts CA, Spector SA Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction. Blood, 2007-09-25;111(1):175-82. 2007-09-25 [PMID: 17895402] (Neutralization, Human)
• Haringman JJ, Smeets TJ, Reinders-Blankert P Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann. Rheum. Dis., 2005-08-17;65(3):294-300. 2005-08-17 [PMID: 16107514] (IHC-Fr, Human)
• Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. Blood, 2004-03-25;104(2):314-20. 2004-03-25 [PMID: 15044249] (ICC, Human)
• Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells. J. Leukoc. Biol., 2004-01-23;75(3):504-14. 2004-01-23 [PMID: 14742635] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCL5