Recombinant Human CCL4/MIP-1 beta Protein

SDS-Page: Recombinant Human CCL4/MIP-1 beta Protein [NBP2-35016]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CCL4/MIP-1 beta Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CCL4/MIP-1 beta Source: E. coli

  Uniprot ID: P13236

  Amino Acid Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN

• Details of Functionality: CCL4/MIP-1 beta protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL4
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL4/MIP-1 beta as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 7.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CCL4/MIP-1 beta Protein

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1beta (MIP-1beta) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV- 1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL4
• Uniprot:
  • P13236()
  • P13236()
  • P13236()