Recombinant Human CCL4/MIP-1 beta Protein Summary
A single, non-glycosylated biologically active polypeptide chain corresponding to 69 residues of CCL4.
Source: E. coli
Amino Acid Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
Macrophage Inflammatory Protein 1 beta Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml
| Protein/Peptide Type
Biologically Active Protein
>96%, by HPLC
Less than 1 EU/ug of endotoxin as determined by LAL method.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
>96%, by HPLC
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human CCL4/MIP-1 beta Protein
- C-C motif chemokine 4
- chemokine (C-C motif) ligand 4
- G-26 T-lymphocyte-secreted protein
- Lymphocyte activation gene 1 protein
- Macrophage inflammatory protein 1-beta
- MIP1 beta
- MIP-1 beta
- PAT 744
- Protein H400
- secreted protein G-26
- small inducible cytokine A4 (homologous to mouse Mip-1b)
- Small-inducible cytokine A4
- T-cell activation protein 2
CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1beta (MIP-1beta) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV- 1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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