CCL4/MIP-1 beta Antibody (24006) [Unconjugated]

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Recombinant HumanCCL4/MIP‑1 beta (Catalog #271-BME) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-ready, ELISA(Cap), Flow
Clone
24006
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

CCL4/MIP-1 beta Antibody (24006) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Ala24-Asn92
Accession # P13236
Specificity
Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, less than 4% cross-reactivity with recombinant human
(rh) CCL3/MIP‑1 alpha and no cross-reactivity with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CCL4
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.3-1.2 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CCL4/MIP-1beta Biotinylated Antibody (Catalog number BAF271)
Standard: Recombinant Human CCL4/MIP-1beta (Catalog number 271-BME)
Publications
Read Publications using
MAB271 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL4/MIP-1 beta Antibody (24006) [Unconjugated]

  • ACT2
  • ACT-2
  • AT744.1
  • C-C motif chemokine 4
  • CCL4
  • chemokine (C-C motif) ligand 4
  • Exodus-3
  • G-26 T-lymphocyte-secreted protein
  • G-26
  • HC21
  • LAG1
  • LAG-1
  • Lymphocyte activation gene 1 protein
  • Macrophage inflammatory protein 1-beta
  • MIP1 beta
  • MIP-1 beta
  • MIP1B
  • MIP1B1
  • MIP-1-beta
  • MIP-1-beta(1-69)
  • PAT 744
  • Protein H400
  • SCYA2
  • SCYA4
  • secreted protein G-26
  • SIS-gamma
  • small inducible cytokine A4 (homologous to mouse Mip-1b)
  • Small-inducible cytokine A4
  • T-cell activation protein 2

Background

CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL4/MIP-1 beta Antibody (MAB271)(16)

We have publications tested in 2 confirmed species: Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 9 applications: Antibody Array Development, ELISA Development, ELISpot Development, Flow Cytometry, ICC, Immunodepletion, Lateral Flow, Luminex Development, Neutralization.


Filter By Application
Antibody Array Development
(1)
ELISA Development
(3)
ELISpot Development
(1)
Flow Cytometry
(1)
ICC
(1)
Immunodepletion
(1)
Lateral Flow
(1)
Luminex Development
(3)
Neutralization
(3)
All Applications
Filter By Species
Human
(14)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using MAB271 Applications Species
A van Hooij, EM Tjon Kon F, M Batista da, R Carvalho B, AC Cunha Mess, AR Gobbo, T Lema, K Bobosha, J Li, X Weng, CG Salgado, JS Spencer, PLAM Corstjens, A Geluk Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia Sci Rep, 2018;8(1):17920. 2018 [PMID: 30560920] (Lateral Flow, Human) Lateral Flow Human
S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection PLoS Pathog., 2018;14(2):e1006888. 2018 [PMID: 29474461]
Kageyama S, Ikeda H, Miyahara Y, Imai N, Ishihara M, Saito K, Sugino S, Ueda S, Ishikawa T, Kokura S, Naota H, Ohishi K, Shiraishi T, Inoue N, Tanabe M, Kidokoro T, Yoshioka H, Tomura D, Nukaya I, Mineno J, Takesako K, Katayama N, Shiku H Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer. Clin Cancer Res, 2015;21(10):2268-77. 2015 [PMID: 25855804] (ELISpot Development, Human) ELISpot Development Human
Cardone M, Ikeda K, Varano B, Gessani S, Conti L HIV-1-induced impairment of dendritic cell cross talk with gammadelta T lymphocytes. J Virol, 2015;89(9):4798-808. 2015 [PMID: 25673717] (Neutralization, Human) Neutralization Human
Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009;69(15):6249-55. 2009 [PMID: 19638592] (ICC, Human) ICC Human
Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009;87(12):1814-20. 2009 [PMID: 19543058] (Antibody Array Development, Human) Antibody Array Development Human
Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes. Clin. Immunol., 2008;128(1):57-65. 2008 [PMID: 18434252] (ELISA Development, Human) ELISA Development Human
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. 2008 [PMID: 18327985] (Luminex Development, Human) Luminex Development Human
Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua. J. Pathol., 2008;214(3):328-36. 2008 [PMID: 18069648] (ELISA Development, Human) ELISA Development Human
Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 2007;117(12):3664-3672. 2007 [PMID: 17992260] (Neutralization, Human) Neutralization Human
Show All 16 Publications.

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Bioinformatics

Gene Symbol CCL4
Uniprot