CCL4/MIP-1 beta Antibody (24006) [Fluorescein]

Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP-1 beta Fluorescein-conjugated Monoclonal Antibody (Catalog # IC271F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 24006
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

CCL4/MIP-1 beta Antibody (24006) [Fluorescein] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
  Ala24-Asn92
  Accession # P13236
• Specificity: Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP‑1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP‑1 alpha , rmCCL4/MIP‑1 beta , rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL4/MIP-1 beta Antibody (24006) [Fluorescein]

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL4/MIP-1 beta Antibody (IC271F)(12)

Publications using IC271F

• Preservation of Lymphopoietic Potential and Virus Suppressive Capacity by CD8+ T Cells in HIV-2-Infected Controllers J Immunol, 2016-08-26;0(0):. 2016-08-26 [PMID: 27566819] (Flow Cytometry, Human)
• van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset. PLoS Pathog, 2015-03-24;11(3):e1004671. 2015-03-24 [PMID: 25803478] (Flow Cytometry, Human)
• Boer M, van Meijgaarden K, Joosten S, Ottenhoff T CD8+ regulatory T cells, and not CD4+ T cells, dominate suppressive phenotype and function after in vitro live Mycobacterium bovis-BCG activation of human cells. PLoS ONE, 2014-04-08;9(4):e94192. 2014-04-08 [PMID: 24714620] (Flow Cytometry, Human)
• Boer M, van Meijgaarden K, Bastid J, Ottenhoff T, Joosten S CD39 is involved in mediating suppression by Mycobacterium bovis BCG-activated human CD8(+) CD39(+) regulatory T cells. Eur J Immunol, 2013-05-29;43(7):1925-32. 2013-05-29 [PMID: 23606272] (Flow Cytometry, Human)
• Ladell K, Hashimoto M, Iglesias M, Wilmann P, McLaren J, Gras S, Chikata T, Kuse N, Fastenackels S, Gostick E, Bridgeman J, Venturi V, Arkoub Z, Agut H, van Bockel D, Almeida J, Douek D, Meyer L, Venet A, Takiguchi M, Rossjohn J, Price D, Appay V A molecular basis for the control of preimmune escape variants by HIV-specific CD8+ T cells. Immunity, 2013-03-21;38(3):425-36. 2013-03-21 [PMID: 23521884] (Flow Cytometry, Human)
• Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope. PLoS Pathog, 2012-11-29;8(11):e1003042. 2012-11-29 [PMID: 23209413] (Flow Cytometry, Human)
• Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure HM, O'Neil SP, Kaur A Early Induction of Polyfunctional Simian Immunodeficiency Virus (SIV)-Specific T Lymphocytes and Rapid Disappearance of SIV from Lymph Nodes of Sooty Mangabeys during Primary Infection. J. Immunol., 2011-03-25;186(9):5151-61. 2011-03-25 [PMID: 21441446] (Flow Cytometry, Primate - Cercocebus atys (Sooty Mangabey))
• Guzzo C, Che Mat NF, Gee K Interleukin-27 induces a STAT1/3- and NF-kappaB-dependent proinflammatory cytokine profile in human monocytes. J. Biol. Chem., 2010-06-02;285(32):24404-11. 2010-06-02 [PMID: 20519510] (Flow Cytometry, Human)
• Reeves RK, Gillis J, Wong FE CD16- natural killer cells: enrichment in mucosal and secondary lymphoid tissues and altered function during chronic SIV infection. Blood, 2010-03-25;115(22):4439-46. 2010-03-25 [PMID: 20339088] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Human)
• Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes. Blood, 2008-01-18;111(7):3579-90. 2008-01-18 [PMID: 18203954] (Flow Cytometry, Human)
• Kivioja J, Rinaldi L, Ozenci V, Kouwenhoven M, Kostulas N, Lindgren U, Link H Chemokines and their receptors in whiplash injury: elevated RANTES and CCR-5. J. Clin. Immunol., 2001-07-01;21(4):272-7. 2001-07-01 [PMID: 11506197] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCL4
• Uniprot:
  • P13236()