Recombinant Mouse CCL3/MIP-1 alpha Protein

SDS-Page: Mouse CCL3/MIP-1 alpha Protein [NBP2-35193]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CCL3/MIP-1 alpha Protein Summary

• Description: A recombinant protein containing 69 amino acid residues corresponding to CCL3/MIP-1 alpha, including the four highly conserved cysteine residues present in CC chemokines Source: E. coli

  Uniprot ID: P10855

  Amino Acid Sequence: APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA

• Details of Functionality: CCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using murine splenocytes is in a concentration range of 10-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL3
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of CCL3/MIP-1 alpha as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 7.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CCL3/MIP-1 alpha Protein

• C-C motif chemokine 3
• MIP1-(a)
• AI323804
• CCL3
• chemokine (C-C motif) ligand 3
• G0S19-1
• LD78a
• LD78alpha
• MIP1 alpha
• MIP-1 alpha
• MIP-1 alpha/CCL3
• MIP1A
• MIP-1alpha
• MIP1-alpha
• PAT 464.1
• SCYA3
• SIS-beta

Background

CCL3, also known as macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1alpha can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant murine MIP-1alpha is a 7.9 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and mature mouse CCL3 shares 73 %, 91 % and 82 % amino acid sequence identity with human, rat and cotton rat CCL3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CCL3/MIP-1 alpha Recombinant Protein (NBP2-35193)(1)

Publication using NBP2-35193

• Guo X, Pan Y, Xiong M et al. Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth Nat Commun 2020-05-01 [PMID: 32358581] (Mouse)

Bioinformatics

• Gene Symbol: CCL3
• Uniprot:
  • P10855()
  • P10855()
  • P10855()