CCL3/MIP-1 alpha Antibody [Alexa Fluor® 405]

• Reactivity: Cr
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

CCL3/MIP-1 alpha Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant cotton rat CCL3/MIP-1α
  Ala24-Ala92
  Accession # AAL26704
• Specificity: Detects cotton rat CCL3/MIP‑1 alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MIP-1 alpha is observed, 15% cross-reactivity with recombinant human (rh) MIP-1 alpha is observed, les
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/MIP-1 alpha Antibody [Alexa Fluor® 405]

• C-C motif chemokine 3
• MIP1-(a)
• AI323804
• CCL3
• chemokine (C-C motif) ligand 3
• G0S19-1
• LD78a
• LD78alpha
• MIP1 alpha
• MIP-1 alpha
• MIP1A
• MIP-1alpha
• MIP1-alpha
• PAT 464.1
• SCYA3
• SIS-beta

Background

MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and MIP-1 beta , two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat MIP-1 alpha cDNA encodes a 92 amino acid (aa) residue precursor protein with a 23 aa putative signal peptide. Mature cotton rat MIP-1 alpha shares approximately 70% amino acid identity with human MIP-1 alpha . MIP-1 alpha is expressed in a wide variety of cells, including lymphocytes, fibroblasts, and epithelial cells, as well as monocytes/macrophages.

MIP-1 alpha has been shown to play an important role in the recruitment of mononuclear cells. Additionally, MIP-1 alpha has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. MIP-1 alpha has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, MIP-1 alpha augments cytolytic activity of NK cells (1). MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that MIP-1 alpha can bind the chemokine receptors CCR1 and CCR5 (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.