CCL3/CCL4 Antibody (93321) [Unconjugated]

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Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF
Clone
93321
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

CCL3/CCL4 Antibody (93321) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha
Ala27-Ala92
Accession # P10147
Specificity
Detects human CCL3/MIP-1 alpha in direct ELISAs and Western blots. In direct ELISAs, greater than 75% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta is observed while approximately 30% cross-reactivity with recombinant mouse (rm) CCL3, rmCCL4/MIP-1 beta, and rhCCL14 is observed. No cross-reactivity with rhCCL1, 2, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, rmCCL1, 2, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL3
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.075-0.375 ug/mL
Publications
Read Publications using
MAB270 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/CCL4 Antibody (93321) [Unconjugated]

  • CCL3/CCL4

Background

The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP‑1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP‑1 alpha. Mature MIP-1 alpha and MIP‑1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP‑1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP‑1 alpha and MIP‑1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP‑1 alpha has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP‑1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP‑1 alpha has been identified as CCR1 and CCR5.
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL3/CCL4 Antibody (MAB270)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Co-Immunoprecipitation, ELISpot Development, ICC, IHC, IHC-Fr, Neutralization.


Filter By Application
Co-Immunoprecipitation
(1)
ELISpot Development
(1)
ICC
(1)
IHC
(1)
IHC-Fr
(1)
Neutralization
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB270 Applications Species
W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation Nat Commun, 2018;9(1):5127. 2018 [PMID: 30510188]
S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection PLoS Pathog., 2018;14(2):e1006888. 2018 [PMID: 29474461] (Neutralization, Human) Neutralization Human
Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 0;97(0):133-140. 0 [PMID: 28648867] (IHC-Fr, Human) IHC-Fr Human
HM Baldwin, MD Singh, V Codullo, V King, H Wilson, I McInnes, GJ Graham Elevated ACKR2 expression is a common feature of inflammatory arthropathies Rheumatology (Oxford), 2017;0(0):. 2017 [PMID: 28486662] (IHC, Human) IHC Human
Kanegasaki S, Matsushima K, Shiraishi K, Nakagawa K, Tsuchiya T Macrophage inflammatory protein derivative ECI301 enhances the alarmin-associated abscopal benefits of tumor radiotherapy. Cancer Res, 2014;74(18):5070-8. 2014 [PMID: 25038226] (Co-Immunoprecipitation, Mouse) Co-Immunoprecipitation Mouse
Snyder-Cappione JE, Tincati C, Eccles-James IG, Cappione AJ, Ndhlovu LC, Koth LL, Nixon DF A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-alpha and MIP1-beta, a lack of IL-17, and a Th1-bias in males. PLoS ONE, 2010;5(11):e15412. 2010 [PMID: 21082024] (ELISpot Development, Human) ELISpot Development Human
Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009;69(15):6249-55. 2009 [PMID: 19638592] (ICC, Human) ICC Human
Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H Induction of host chemotactic response by Encephalitozoon spp. Infect. Immun., 2007;75(4):1619-25. 2007 [PMID: 17178789] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol CCL3
Uniprot