CCL26/Eotaxin-3 Antibody [Unconjugated]

Images

 
Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346‑E3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL‑4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CCL26/Eotaxin-3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL26/Eotaxin‑3
Thr24-Leu94
Accession # Q9Y258
Specificity
Detects CCL26/Eotaxin‑3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Eotaxin, recombinant mouse (rm) Eotaxin, rhEotaxin-2, and rmEotaxin-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL26
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 10-25 ug/mL
Publications
Read Publications using
AF653 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL26/Eotaxin-3 Antibody [Unconjugated]

  • CC chemokine IMAC
  • C-C motif chemokine 26
  • CCL26
  • chemokine (C-C motif) ligand 26
  • chemokine N1
  • Eotaxin3
  • Eotaxin-3
  • IMAC
  • Macrophage inflammatory protein 4-alpha
  • MGC126714
  • MIP-4a
  • MIP-4alpha
  • SCYA26
  • SCYA26MIP-4-alpha
  • small inducible cytokine A26
  • small inducible cytokine subfamily A (Cys-Cys), member 26
  • Small-inducible cytokine A26
  • TSC-1Thymic stroma chemokine-1

Background

Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin‑3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4.

Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Recombinant Eotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.

  1. Gou, R.F. et al. (1999) Genomics 58:313.
  2. Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
  3. Shinkai, A. et al. (1999) J. Immunol. 163:1602.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL26/Eotaxin-3 Antibody (AF653)(3)

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Bioinformatics

Gene Symbol CCL26
Uniprot