CCL26/Eotaxin-3 Antibody (115002) [Unconjugated]

Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL26/ Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346‑E3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin‑3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin‑3 Monoclonal Antibody (Catalog # MAB653). The ND50 is typically 2-8 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 115002
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL26/Eotaxin-3 Antibody (115002) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL26/Eotaxin-3
  Thr24-Leu94
  Accession # Q9Y258
• Specificity: Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CCL11/Eotaxin, recombinant mouse (rm) CCL11/Eotaxin, rhCCL24/Eotaxin-2, rmCCL24/Eotaxin-2, rhCCL3, rhCCL4, rmCCL3, rmCCL4, rhCCL5, rhCCL13, or rhCCL14.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL26
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 2-8 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: ELISA Detection: Human CCL26/Eotaxin-3 Biotinylated Antibody (Catalog number BAF346)
  Standard: Recombinant Human CCL26/Eotaxin-3 (Catalog number 653-E3)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL26/Eotaxin-3 Antibody (115002) [Unconjugated]

• CC chemokine IMAC
• C-C motif chemokine 26
• CCL26
• chemokine (C-C motif) ligand 26
• chemokine N1
• Eotaxin3
• Eotaxin-3
• IMAC
• Macrophage inflammatory protein 4-alpha
• MGC126714
• MIP-4a
• MIP-4alpha
• SCYA26
• SCYA26MIP-4-alpha
• small inducible cytokine A26
• small inducible cytokine subfamily A (Cys-Cys), member 26
• Small-inducible cytokine A26
• TSC-1Thymic stroma chemokine-1

Background

CCL26, also known as Eotaxin-3, is a CC chemokine that functions as a chemoattractant for eosinophils and activated T cells through interactions with CCR3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL26/Eotaxin-3 Antibody (MAB653)(3)

Publications using MAB653

• Cynthia Hajal, Yoojin Shin, Leanne Li, Jean Carlos Serrano, Tyler Jacks, Roger D. Kamm The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain Science Advances 2021-06-25 [PMID: 34162553]
• Miyagaki T, Sugaya M, Fujita H Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma. J. Invest. Dermatol., 2010-05-27;130(9):2304-11. 2010-05-27 [PMID: 20505746] (IHC-P, Human)
• Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. Blood, 2004-03-25;104(2):314-20. 2004-03-25 [PMID: 15044249] (ELISA Development, ICC, Human)

Bioinformatics

• Gene Symbol: CCL26
• Uniprot:
  • Q9Y258()