CCL26/Eotaxin-3 Antibody (115002) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: WB, B/N, ELISA, ELISA(Cap), ELISA(Det)
• Clone: 115002
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

CCL26/Eotaxin-3 Antibody (115002) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human CCL26/Eotaxin-3
  Thr24-Leu94
  Accession # Q9Y258
• Specificity: Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CCL11/Eotaxin, recombinant mouse (rm) CCL11/Eotaxin, rhCCL24/Eotaxin-2, rmCCL24/Eotaxin-2, rhCCL3, rhCCL4, rmCCL3, rmCCL4, rhCCL5, rhCCL13, or rhCCL14.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL26
• Purity: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Neutralization
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CCL26/Eotaxin-3 Antibody (115002) [CoraFluor™ 1]

• CC chemokine IMAC
• C-C motif chemokine 26
• CCL26
• chemokine (C-C motif) ligand 26
• chemokine N1
• Eotaxin3
• Eotaxin-3
• IMAC
• Macrophage inflammatory protein 4-alpha
• MGC126714
• MIP-4a
• MIP-4alpha
• SCYA26
• SCYA26MIP-4-alpha
• small inducible cytokine A26
• small inducible cytokine subfamily A (Cys-Cys), member 26
• Small-inducible cytokine A26
• TSC-1Thymic stroma chemokine-1

Background

Eotaxin-3, also known as CCL26, is one of two cytokine genes grouped on the q arm of chromosome 7. As part of the Cytokines family of secreted proteins, Eotaxin-3 is involved in the immunoregulatory and inflammatory process. This gene shows chemotactic activity for peripheral blood eosinophils and basophils and also binds to the chemokine receptor CCR3. It also contributes to the eosinophil accumulation in atopic diseases such as asthma and atopic dermatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL26