CCL25/TECK Antibody (89827) [Unconjugated]

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Cap)
• Clone: 89827
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL25/TECK Antibody (89827) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL25/TECK
  Gln24-Asn144
  Accession # O35903.1
• Specificity: Detects mouse CCL25/TECK in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9, 11, 12, 19, 20, 21, 22, 24, and recombinant rat CCL20 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL25
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse CCL25/TECK Biotinylated Antibody (Catalog number BAF481)
  Standard: Recombinant Mouse CCL25/TECK (Catalog number 481-TK)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL25/TECK Antibody (89827) [Unconjugated]

• C-C motif chemokine 25
• CCL25
• chemokine (C-C motif) ligand 25
• Chemokine TECK
• Ck beta-15
• Ckb15
• SCYA25TECKvar
• small inducible cytokine subfamily A (Cys-Cys), member 25
• Small-inducible cytokine A25
• TECK
• TECKMGC150327
• thymus expressed chemokine
• Thymus-expressed chemokine

Background

CCL25 (thymus-expressed chemokine), also known as TECK (thymus-expressed chemokine), is a CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein.  Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. CCL25 functions through interactions with CCR9 and is chemotactic for activated macrophages, dendritic cells and thymocytes (1).

1. Vicari, A.P. et al. (1997) Immunity 7:291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL25/TECK Antibody (MAB4811)(3)

Publications using MAB4811

• Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction. Eur. J. Immunol., 2012-05-01;42(5):1250-60. 2012-05-01 [PMID: 22539297] (ELISA Development, Mouse)
• Ericsson A, Kotarsky K, Svensson M, Sigvardsson M, Agace W Functional characterization of the CCL25 promoter in small intestinal epithelial cells suggests a regulatory role for caudal-related homeobox (Cdx) transcription factors. J. Immunol., 2006-03-15;176(6):3642-51. 2006-03-15 [PMID: 16517733] (IHC-Fr, Mouse)
• Misslitz A, Pabst O, Hintzen G, Ohl L, Kremmer E, Petrie HT, Forster R Thymic T cell development and progenitor localization depend on CCR7. J. Exp. Med., 2004-08-09;200(4):481-91. 2004-08-09 [PMID: 15302903] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CCL25
• Uniprot:
  • O35903.1()