CCL25/TECK Antibody (52529) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
52529
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

CCL25/TECK Antibody (52529) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL25/TECK
Gln24-Leu150
Accession # AAB69981
Specificity
Detects human CCL25/TECK in direct ELISAs and Western blots. Shows less than 1% cross-reactivity with recombinant mouse CCL25 and recombinant rat CINC-1 and no cross-reactivity with recombinant human (rh)CCL28, recombinant mouse (rm)CCL28, rhMPIF-1, rh6Ckine, rm6Ckine, rhEotaxin, or rmEotaxin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL25
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CCL25/TECK Biotinylated Antibody (Catalog number BAF334)
Standard: Recombinant Human CCL25/TECK (Catalog number 334-TK)
Reviewed Applications
Read 1 Review rated 5
using
MAB3341 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL25/TECK Antibody (52529) [Unconjugated]

  • C-C motif chemokine 25
  • CCL25
  • chemokine (C-C motif) ligand 25
  • Chemokine TECK
  • Ck beta-15
  • Ckb15
  • SCYA25TECKvar
  • small inducible cytokine subfamily A (Cys-Cys), member 25
  • Small-inducible cytokine A25
  • TECK
  • TECKMGC150327
  • thymus expressed chemokine
  • Thymus-expressed chemokine

Background

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 127 residue mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 amino acid protein that exhibits 49% amino acid sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 has also been shown to be a specific agonist for the orphan receptor called GPR-9-6, now named CCR9.

  1. Vicari, A.P. et al. (1997) Immunity 7:291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for CCL25/TECK Antibody (MAB3341) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Pig.

Reviews using MAB3341:
Filter by Applications
IHC-P
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CCL25/TECK MAB3341
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5
reviewed by:
Anonymous
IHC-P Pig 09/04/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMammary gland tissue
SpeciesPig
Lotdnt021908a

Comments

Comments10 ug/ml of the primary antibody was used for staining. Antigen retrieval was done by a microwave method in citric acid (pH 6.0). Detection of the mouse antibody was done by the anti-mouse antibody-Alex 488 (1:200 dilution).

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Anonymous
09/04/2019
Application: IHC-P
Species: Pig

Bioinformatics

Gene Symbol CCL25
Uniprot