E. coli-derived recombinant human CCL25/TECK Gln24-Leu150 Accession # AAB69981
Detects human CCL25/TECK in direct ELISAs and Western blots. Shows less than 1% cross-reactivity with recombinant mouse CCL25 and recombinant rat CINC-1 and no cross-reactivity with recombinant human (rh)CCL28, recombinant mouse (rm)CCL28, rhMPIF-1, rh6Ckine, rm6Ckine, rhEotaxin, or rmEotaxin.
Protein A or G purified from ascites
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CCL25/TECK Antibody (52529) [Unconjugated]
C-C motif chemokine 25
chemokine (C-C motif) ligand 25
small inducible cytokine subfamily A (Cys-Cys), member 25
Small-inducible cytokine A25
thymus expressed chemokine
CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 127 residue mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 amino acid protein that exhibits 49% amino acid sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 has also been shown to be a specific agonist for the orphan receptor called GPR-9-6, now named CCR9.
Vicari, A.P. et al. (1997) Immunity 7:291.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
10 ug/ml of the primary antibody was used for staining. Antigen retrieval was done by a microwave method in citric acid (pH 6.0). Detection of the mouse antibody was done by the anti-mouse antibody-Alex 488 (1:200 dilution).
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